We narrowed to 24,854 results for: Spr

1. pST_116_LVL2 cam

   Plasmid

   #179332

   Purpose

   NT-CRISPR plasmid for a single gRNA.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT3TS nErCas12an

   Plasmid

   #132642

   Purpose

   Used for in vitro transcription of dual NLS ErCas12a mRNA

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   CRISPR; In vitro transcription

   Tags

   SV40 NLS

   Promoter

   T3

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST_119_LVL2 cam

   Plasmid

   #179333

   Purpose

   NT-CRISPR plasmid for integration of multiple gRNAs.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
   

   Use

   CRISPR

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSL0284 (pQCascade_entry)

   Plasmid

   #130635

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPN461

   Plasmid

   #137877

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   POGZ (POGZ Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPN460

   Plasmid

   #137878

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   POGZ (POGZ Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPN455

   Plasmid

   #137872

   Purpose

   Expression of gRNA targeting POGZ for CRISPRa; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   POGZ (POGZ Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPN447

   Plasmid

   #137871

   Purpose

   Expression of gRNA targeting POGZ for CRISPRi; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   POGZ (POGZ Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ262-ABE8e

   Plasmid

   #161523

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   ABE8e-zSpCas9(D10A)
   

   Use

   CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSCIP-hCD69-TdTomato

    Plasmid

    #154095

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Inserts

    [5HA]-P2A-tdTomato-[3HA]
    hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ262m

    Plasmid

    #161522

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPN441

    Plasmid

    #137873

    Purpose

    PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPN440

    Plasmid

    #137874

    Purpose

    PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPN458

    Plasmid

    #137876

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPN432

    Plasmid

    #137870

    Purpose

    Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN431

    Plasmid

    #137869

    Purpose

    Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge