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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,044 results for: cloning

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Showing: 1281 - 1300 of 63044 results

  1. pcDNA3-C-terminal GST

    Plasmid
    #128025
    Purpose
    Mammalian expression vector with C terminal GST tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    GST
    Expression
    Mammalian
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128025-plasmid-map-sequence-id-254769

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  2. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Expression
    Yeast
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    83476-plasmid-map-sequence-id-189195

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  6. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    58807-plasmid-map-sequence-id-91105

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  8. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128059-plasmid-map-sequence-id-256675

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  11. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  16. pCAP01

    Plasmid
    #59981
    Purpose
    The function of this material is to facilitate the capture and expression of bacterial gene clusters.
    Depositor
    Bradley Moore
    Publication
    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111
    Type
    Empty backbone
    Use
    Synthetic Biology; Transformation-associated reco…
    Tags
    no
    Expression
    Bacterial and Yeast
    Promoter
    no
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    59981-plasmid-map-sequence-id-96247

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Showing: 1281 - 1300 of 63044 results