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pGLOW30

Plasmid

#172697

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-2

Available Since

Sept. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L4440_BioBrick-hsf-1-sgRNA-A

Plasmid

#177786

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGLOW79

Plasmid

#177339

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-3

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo_pAS32

Plasmid

#118222

Purpose

expresses 3xHA-tagged miniTurbo in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-B

Plasmid

#177787

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159872

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159873

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-A

Plasmid

#177784

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Plasmid

#159807

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDD315 (mTurquoise2^SEC^2xHA)

Plasmid

#73343

Purpose

mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

Insert

mTurquoise2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans codon-optimized mTurquoise2

Expression

Worm

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata4

Plasmid

#41084

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Sall4

Plasmid

#40797

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Sall4 (Sall4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Dmrt1

Plasmid

#41083

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Dmrt1 (Dmrt1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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