Addgene

We narrowed to 87,637 results for: lias

Showing: 1281 - 1300 of 87637 results

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pCAG-R2Tg-3xFlag

Plasmid

#223250

Purpose

Mammalian expression of C-terminally Flag-tagged R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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CD8a

Plasmid

#86050

Purpose

A mammalian expression plasmid encoding CD8a without tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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CD8a-EGFP

Plasmid

#86051

Purpose

A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AU1-mTOR-E2419K

Plasmid

#19994

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-E2419K (Mtor Rat)

Use

Tags

AU1

Expression

Mammalian

Mutation

Changed Glutamine 2419 to Lysine

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-Myc:P4:gs:nls:ABI

Plasmid

#135985

Purpose

Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the ABI domain

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223252

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-SRGAP1

Plasmid

#187269

Purpose

Expression of SRGAP1-EGFP

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

SRGAP1 (SRGAP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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XLone-SOX18

Plasmid

#163822

Purpose

expresses human SOX18 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

SOX18 (SOX18 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Use

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-LOx-IRES-EGFP

Plasmid

#166877

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9

Plasmid

#60551

Purpose

Expresses CibN-dCas9 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

CibN-dCas9

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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35S::Cel1SP-GeNL/pCAMBIA1301

Plasmid

#190069

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Cel1 signal peptide-Green-enhanced Nano-lantern

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-BD-cop1at(335-675)

Plasmid

#44973

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

BD-COP1 (COP1 Mustard Weed)

Use

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-B3

Plasmid

#31707

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-20b (Mir20b Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-B2

Plasmid

#31706

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-18b (Mir18b Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
OSCA1.2-K435L-L438K-peGFP-N1

Plasmid

#228587

Purpose

Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

K435L, L438K

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMamCol1

Plasmid

#215184

Purpose

Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

Depositor

Krishna Kishore Inampudi

Insert

tsPurple

Use

AAV, CRISPR, and Synthetic Biology ; Recombinant …

Tags

SNAC-StrepII tag

Expression

Bacterial and Mammalian

Mutation

Promoter

TetON 3G Bidirectional for Mammalian and Trc with…

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-B5

Plasmid

#31709

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-363 (Mir363 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only