We narrowed to 3,177 results for: FRI

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pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

Plasmid

#55199

Purpose

CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

ECFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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NHA-TNGW1

Plasmid

#36437

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

Full length (aa1-1962)

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only
pAP118

Plasmid

#105495

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

HupA-SmBiT/HupA-LgBiT

Use

Atc-dependent expression in c. difficile

Promoter

Ptet

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-PIWI

Plasmid

#11591

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Mutation

contain only aa482-869

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
PliaI

Plasmid

#55177

Purpose

Bacitracin-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

liaI promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

liaI

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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gCOTS-pyl

Plasmid

#92048

Purpose

This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

Depositor

Lital Alfonta

Article

Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

Inserts

Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)

Use

Cyanobacteria s. elongatus genome recombination i…

Promoter

LeuP (native S. elongatus promoter) and PrcbL

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFN_L-HiBiT

Plasmid

#198319

Purpose

Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFN_L-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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PliaG

Plasmid

#55174

Purpose

Constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

LiaG promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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RS18960_pMQ30_KO_Kan

Plasmid

#185393

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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OVA-miSFIT-8-G

Plasmid

#124675

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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RS17060_pMQ30_KO_Kan

Plasmid

#185396

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17055_pMQ30_KO_Kan

Plasmid

#185398

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV TBG FFluc miR122sponge

Plasmid

#35657

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

miR-122 sponge

Use

AAV

Promoter

TBG

Available Since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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J23101

Plasmid

#55176

Purpose

Very weak constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

J23101 promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

J23101

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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NHA-Delta 12

Plasmid

#36439

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 12 (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 896-1219 only

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
NHA- Delta 5

Plasmid

#36440

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 5 (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 1670-1962 only

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Article

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Mutation

T17N. Dominant negative.

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
pRRG43-sFRP1

Plasmid

#99071

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

sFRP1 (secreted frizzled related protein 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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