Addgene

We narrowed to 26,848 results for: Tat

Showing: 1301 - 1320 of 26848 results

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PADH1-TIR1-9myc-URA3

Plasmid

#207016

Purpose

Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

TIR1

Use

Tags

9xMyc

Expression

Yeast

Mutation

Promoter

pADH1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

Plasmid

#207031

Purpose

Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Use

Tags

mRuby3

Expression

Yeast

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

Plasmid

#207024

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

D384A Y387A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

Plasmid

#207023

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

W371A I375A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

Plasmid

#207022

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

Y387A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A-GFP

Plasmid

#207021

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

D384A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-I375A-GFP

Plasmid

#207020

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

I375A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A-GFP

Plasmid

#207019

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Use

Tags

GFP

Expression

Yeast

Mutation

W371A

Promoter

pTEF1

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7repeats)-GFP-Pho8

Plasmid

#207011

Purpose

Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Ypq2-2GFP-URA

Plasmid

#207010

Purpose

Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

YPQ2

Use

Tags

2xGFP

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Vph1-2GFP-URA

Plasmid

#207009

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

VPH1

Use

Tags

2xGFP

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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p4A8_M59I_T94M_BC

Plasmid

#217833

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Use

Tags

Expression

Yeast

Mutation

VH: M59I, VL: T94M

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBDP

Plasmid

#217827

Purpose

Stuffer plasmid with golden gate compatible bidirectional promoter

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

Gal1/Gal10 Bidirectional Promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8-MET15

Plasmid

#207055

Purpose

Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

Promoter

pATG8

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆R-MET15

Plasmid

#207034

Purpose

Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xHA

Expression

Yeast

Mutation

∆R

Promoter

pATG8

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IRdist_scram

Plasmid

#162319

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

The distal Upstream Motif site is scrambled

Promoter

pBAD

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1954

Plasmid

#195689

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A/L123A)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2319

Plasmid

#195697

Purpose

Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

Foldon-MlaC

Use

Tags

Expression

Bacterial

Mutation

6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2707

Plasmid

#195636

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Use

Tags

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251)

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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