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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)
Plasmid#171100PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.DepositorInserthSpCas9 (Neurog2 Mouse, S. pyogenes)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)
Plasmid#171099PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.DepositorInserthSpCas9 (Ascl1 Mouse, S. pyogenes)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTALYM4
Plasmid#47875PurposeDestination plasmid with mRuby2 for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALYM9
Plasmid#47876PurposeDestination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsTy1tagExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALYM3
Plasmid#47874PurposeDestination plasmid with mClover for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmCloverExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-p15
Plasmid#62656PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmidDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)
Plasmid#208289PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA; mutations in iSpyMacPromoterCMV and T7Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
MALT1 (3K0W)
Plasmid#25306PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.129.326 (MALT1 Human)
UseTagsHisExpressionBacterialMutationamino acid residues 128-326PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MALT1 (3BFO)
Plasmid#25216PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.226.314 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 226-314 of Isoform 2PromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4.23 C3_21
Plasmid#60296PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorInsertCDKN2BAS enhancer (CDKN2B-AS1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-PEmax-P2A-EGFP (LM1589)
Plasmid#223136PurposepCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFPDepositorInserthuman codon optimized PEmax-P2A-EGFP
UseCRISPRTagsBPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFPExpressionMammalianMutationM-MLV mutations from PE2 (Anzalone et al. Nature …PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)
Plasmid#223110PurposepCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2DepositorInserthuman codon optimized nuclease SpG-P2A-mTagBFP2
UseCRISPRTagsBPNLS-3xFLAG-P2A-mTagBFP2ExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
MALT1-DD (2G7R)
Plasmid#25613PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMALT1-DD (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains Death DomainPromoterAvailable sinceAug. 31, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGX-5Dual
Plasmid#226869Purposetransient expressionDepositorInsertLuciferase and hRluc
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only
