Addgene

We narrowed to 3,042 results for: AER

Showing: 1321 - 1340 of 3042 results

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Use

Tags

Expression

Mammalian

Mutation

KO of both AP1 sites

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIF2_invivo

Plasmid

#196402

Purpose

expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IF2 protein (orf30 P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1

Plasmid

#203835

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Use

Tags

Expression

Mammalian

Mutation

KO of AP1 upstream

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP2

Plasmid

#203836

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Use

Tags

Expression

Mammalian

Mutation

KO of AP1 downstream

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProC and no promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProD

Plasmid

#198431

Purpose

mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ProD and no promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-eDHFR

Plasmid

#194635

Purpose

Mammalian expression of the eDHFR control bait in Virotrap

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Use

Tags

ecDHFR

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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MPC021: CamKII CMV_sNovArch_citrine (soma targeting)

Plasmid

#153194

Purpose

encoded soma targeted NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Use

Tags

citrine

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-2

Plasmid

#160702

Purpose

gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

gpdh

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS427-pCSDest2-AcrE2

Plasmid

#85677

Purpose

Mammalian expression of anti-CRISPR protein from Type I-E

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type I-E anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.9

Plasmid

#78179

Purpose

Expresses-rsGreen0.9 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.9

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7b

Plasmid

#78178

Purpose

Expresses rsGreen0.7b in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7b

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00582

Plasmid

#39666

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5369 (CG5369 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

AprR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aalb_743

Plasmid

#176676

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-HaloCaMP1a-EGFP

Plasmid

#138327

Purpose

chemigenetic calcium indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Has Service

AAV1 and AAV9

Insert

HaloCaMP1a

Use

AAV

Tags

EGFP, His-tag, and Nuclear Exclusion Signal

Expression

Mammalian

Mutation

Promoter

synapsin

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

SmR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM2-Halo-FRB

Plasmid

#197056

Purpose

Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pORTMAGE312B

Plasmid

#128969

Purpose

Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E32K

Use

Tags

Expression

Bacterial

Mutation

E32K

Promoter

xylS-Pm

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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