We narrowed to 7,184 results for: Ank

1. mCherry-eDHFR

   Plasmid

   #107266

   Purpose

   mCherry-eDHFR in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR (dhfr E.coli)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDA056

   Plasmid

   #131387

   Purpose

   ORFeus expression, WT

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   ORFeus-Hs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBac{3XP3::EYFP,attP}

   Plasmid

   #86860

   Purpose

   Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

   Insert

   attP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0044-EF1a-RanCas13b-NES-mapk

   Plasmid

   #103855

   Purpose

   Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   RanCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and mapk NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-HA-SZT2

   Plasmid

   #87035

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-TCF7

   Plasmid

   #79498

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB22

   Plasmid

   #103059

   Purpose

   medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp

   Depositor

   Dirk Bumann

   Article

   Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

   Insert

   GFP-OVA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   hmpAp

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbS8c-ddcpf1-Δ

   Plasmid

   #153039

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP FRNK

   Plasmid

   #50518

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   truncated (NT2186-3268)

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pRK5-FLAG-SZT2

    Plasmid

    #87034

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC025

    Plasmid

    #227597

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid

    #103867

    Purpose

    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    W85X targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MTK4_002

    Plasmid

    #123811

    Purpose

    Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SV40 pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK2_007

    Plasmid

    #123702

    Purpose

    Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pEF1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIPZ TREX1-KI

    Plasmid

    #127701

    Purpose

    For knocking in TREX1 in mouse - Doxycyclin inducible

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    mouse TREX1 cDNA (Trex1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S129A

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-fDIO-stGtACR2-FusionRed

    Plasmid

    #217975

    Purpose

    AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

    Depositor

    Edward Callaway

    Article

    Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

    Insert

    stGtACR2-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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