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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9N

Plasmid

#80934

Purpose

Plasmid that expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-AkCas12b-2AeGFP

Plasmid

#121946

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-BsCas12b-2AeGFP

Plasmid

#121948

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AmCas12b-2AeGFP

Plasmid

#121947

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-TGFβRII-HA

Plasmid

#37040

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

Insert

TGFβRII

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMRX-IP TFEB-sfGFP

Plasmid

#135402

Purpose

Expresses TFEB-sfGFP

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

TFEB (TFEB Human)

Use

Retroviral

Tags

sfGFP

Expression

Mammalian

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i2-T2A-EGFP

Plasmid

#188270

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XmCherry

Plasmid

#64108

Purpose

Expression of Sp dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XmCherry

Plasmid

#64110

Purpose

Expression of Nm dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6b

Plasmid

#215830

Purpose

Express CBE6b (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N

Plasmid

#80930

Purpose

Expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGAMA-empty

Plasmid

#74755

Purpose

Mammalian lentiviral vector

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9C

Plasmid

#80931

Purpose

Expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9FL-P2A-turboGFP

Plasmid

#80941

Purpose

Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9FL

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21

Plasmid

#83421

Purpose

Mouse Rab21 (wild type)

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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