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Plasmid#127497PurposeExpresses myc-tagged NDUFS2/E57A in mammalian cellsDepositorInsertNDUFS2 E57A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 E57APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 T58A/myc-His
Plasmid#127498PurposeExpresses myc-tagged NDUFS2/T58A in mammalian cellsDepositorInsertNDUFS2 T58A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 T58APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 H60A/myc-His
Plasmid#127499PurposeExpresses myc-tagged NDUFS2/H60A in mammalian cellsDepositorInsertNDUFS2 H60A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 H60APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 W61A/myc-His
Plasmid#127500PurposeExpresses myc-tagged NDUFS2/W61A in mammalian cellsDepositorInsertNDUFS2 W61A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 W61APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 K62A/myc-His
Plasmid#127501PurposeExpresses myc-tagged NDUFS2/K62A in mammalian cellsDepositorInsertNDUFS2 K62A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 K62APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 P63A/myc-His
Plasmid#127502PurposeExpresses myc-tagged NDUFS2/P63A in mammalian cellsDepositorInsertNDUFS2 P63A (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 P63APromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-ATG101-ATG13 HORMA
Plasmid#99332PurposeMammalian expression of one subcomplex from the Human ULK1 complexDepositorUseTagsGSTExpressionInsectMutationPromoterp10 and pHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMS-dsRed2-WT LMNA-GFP minigene
Plasmid#128230PurposeLamin A splicing reporterDepositorInsertlamin A (LMNA Human)
UseTagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-TR-TTHA
Plasmid#232479PurposeTetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' endDepositorInsertThermus thermophilus HB8
UseTransposon systemTagsDYKDDDDK (FLAG-tag)ExpressionMammalianMutationPromoterCMV-TetOAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE FL NAP1 STOP C' EGFP IRES Blast
Plasmid#179102PurposeExpresses untagged full length NAP1DepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-N'-FLAG-HA-NAP1S318A
Plasmid#195410PurposeExpresses FLAG-HA tagged human NAP1 with S318A point mutationDepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationSerine 318 turned to AlaninePromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-NB
Plasmid#197092PurposePiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiationDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK19-WT-IRES-ZsGreen
Plasmid#68858PurposeExpresses Human CDK19 Wild-TypeDepositorInsertCyclin-Dependent Kinase 19 (CDK19 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-WT-IRES-mCherry
Plasmid#68856PurposeExpresses Human CDK8 Wild-TypeDepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK19-W105M-IRES-ZsGreen
Plasmid#68859PurposeExpresses Human CDK19 W105M MutantDepositorInsertCyclin-Dependent Kinase 19 (CDK19 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-W105M-IRES-mCherry
Plasmid#68857PurposeExpresses Human CDK8 W105M MutantDepositorInsertCyclin-Dependent Kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only