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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1321 - 1340 of 1503 results

  1. AOP-COMP-blac-flag-his

    Plasmid
    #111011
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    1-cys peroxiredoxin (AOP) (PF3D7_0729200 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111011-plasmid-map-sequence-id-224567

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  2. PF3D7_1352500-COMP-blac-flag-his

    Plasmid
    #110969
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    thioredoxin-related protein, putative (PF3D7_1352500 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110969-plasmid-map-sequence-id-224251

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  3. pOP5TT

    Plasmid
    #112613
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His-thioredoxin and Avi
    Expression
    Bacterial
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112613-plasmid-map-sequence-id-220251

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  5. Py-2-Cys-Prx

    Plasmid
    #25575
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-2-Cys-Prx
    Tags
    His
    Expression
    Bacterial
    Available since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pHL1633

    Plasmid
    #62830
    Purpose
    Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Insert
    gfp
    Use
    Synthetic Biology
    Tags
    T710
    Expression
    Bacterial
    Promoter
    pLlacO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. Py-1Cys-PRX

    Plasmid
    #25580
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-1Cys-PRX
    Tags
    His
    Expression
    Bacterial
    Available since
    Oct. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. Pv-2Cys-Prx

    Plasmid
    #25586
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-2Cys-Prx
    Tags
    His
    Expression
    Bacterial
    Available since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pHL671

    Plasmid
    #62825
    Purpose
    Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Insert
    gfp
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Promoter
    pCon
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pHL1017

    Plasmid
    #62828
    Purpose
    Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Publication
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Insert
    gfp
    Use
    Synthetic Biology
    Tags
    m8::glgC leader
    Expression
    Bacterial
    Promoter
    pConNoHind
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. refGFP1_pQE30

    Plasmid
    #48644
    Purpose
    Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP
    Depositor
    David Ron
    Publication
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    refGFP1
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    refGFP C147S, C204Q
    Available since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48644-plasmid-map-sequence-id-70402

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Showing: 1321 - 1340 of 1503 results