Addgene

We narrowed to 10,856 results for: kars;

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FabF (KASII) pet28 N-terminal His Tag

Plasmid

#75017

Purpose

Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

FabF (fabF E. coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only
MS2-adRNA (5'UAG)

Plasmid

#170126

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'UAG)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-UAG site)

Plasmid

#170138

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(UAG)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Human, Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-p38 (Mapk14 Human, Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q, N496F)

Plasmid

#170139

Purpose

AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-ADAR2-DD(E488Q, N496F)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G20-R20

Plasmid

#101822

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo

Plasmid

#80032

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-N

Plasmid

#170153

Purpose

AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-RESCUE-DDN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot10

Plasmid

#79254

Purpose

Expresses EGFP-tagged DmNot10 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot10 (Not10 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot11

Plasmid

#79253

Purpose

Expresses EGFP-tagged DmNot11 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot11 (Not11 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v1 puro

Plasmid

#58706

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Eco

Plasmid

#106276

Purpose

E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEcoCascade-IL1RNcrRNA

Plasmid

#126481

Purpose

Encodes E.coli CRISPR Type I-E crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Eco IL1RN cr26

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only