We narrowed to 7,837 results for: puc

1. tagRFP-IMBB4-pUC

   Plasmid

   #170769

   Purpose

   test plasmid for 5RM assembly, encodes fluorescent red protein

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Insert

   tagRFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-POLBHR-eGFP

   Plasmid

   #176064

   Purpose

   Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   EGFP-PolB (POLB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   pUC with endogenous SapI mutated out, PAM mutatio…

   Promoter

   N/A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCHR-mClover-MT-C34

   Plasmid

   #177153

   Purpose

   Lentiviral vector for expression of anti-HIV-1 MT-C34 peptide on cell surface

   Depositor

   Dmitriy Mazurov

   Article

   Maslennikova et al mBio. 2022 Jan 25:e0358921. doi: 10.1128

   Insert

   mClover-MT-C34
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-PpSEC13-mEGFP

   Plasmid

   #160469

   Purpose

   P. pastoris integration vector for the gene replacement of SEC13 fused to mEGFP

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   ppSEC13-mEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-PpSEC13-msGFP2

   Plasmid

   #160470

   Purpose

   P. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   ppSEC13-msGFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC_h7SK M15 (CUA)

   Plasmid

   #154784

   Purpose

   plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G10A, G14U, G16U, C20U, C52A

   Promoter

   h7SK

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC_h7SK M15 (UUA)

   Plasmid

   #154785

   Purpose

   plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   UUA anticodon and G10A, G14U, G16U, C20U, C52A

   Promoter

   h7SK

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-DEST-R1R4

   Plasmid

   #141054

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-DEST-R5R4

    Plasmid

    #141055

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-hsg(G:H)

    Plasmid

    #138259

    Purpose

    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mCherry targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. puC19 preACT1(WTdel6)

    Plasmid

    #111324

    Purpose

    for T7 transcription of wt actin pre-mRNA (del6)

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. puC19 preACT1(AcAc)

    Plasmid

    #111327

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    3' splice site AcAc mutation in intron

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. puC19 preACT1(BrG)

    Plasmid

    #111326

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    branch site A to G mutation in intron

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. puC19 preACT1(BrC)

    Plasmid

    #111325

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    branch site A to C mutation in intron

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19 gene 60 to gap

    Plasmid

    #49158

    Purpose

    Template for generating gene 60 mRNA, sequence 5' of coding gap only

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Use

    Tags

    Expression

    Mutation

    Coding gap and downstream sequence have been del…

    Promoter

    T7

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SRAcode_in_pUC57_v3(419-999)

    Plasmid

    #49824

    Purpose

    for transcribing SRA nucl 419-999 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. SRAcode_in_pUC57_v3(767-999)

    Plasmid

    #49825

    Purpose

    for transcribing SRA nucl 767-999 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. SRAcode_in_pUC57_v3(419-771)

    Plasmid

    #49823

    Purpose

    for transcribing SRA nucl 419-771 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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