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pBZS-YP_001497965

Plasmid

#198368

Purpose

Express YP_001497965 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001497965 (B769R )

Expression

Bacterial

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_001498157

Plasmid

#198369

Purpose

Express YP_001498157 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001498157 (C075L )

Expression

Bacterial

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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BPTI-21

Plasmid

#195058

Purpose

simplyfied amino acid sequence by alanine scanning

Depositor

Peter Kim, Yutaka Kuroda

Article

Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

Insert

bovine pancreatic trypsin inhibitor

Tags

His tag and trpΔLE leader sequences

Expression

Bacterial

Mutation

21 amino acids are Alanine out of 58 amino acids

Promoter

T7 promotor

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC2

Plasmid

#191857

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GB3270

Plasmid

#193114

Purpose

Random sequence R3 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R3

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GB3269

Plasmid

#193113

Purpose

Random sequence R2 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R2

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GB2815

Plasmid

#193112

Purpose

Random sequence R1 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R1

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GB3272

Plasmid

#193134

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1b.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GB3273

Plasmid

#193135

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1e.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC6

Plasmid

#191861

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC1

Plasmid

#191856

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE32

Plasmid

#90374

Purpose

SP1 - constitutive enhancer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

SP1

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_wt

Plasmid

#149386

Purpose

P. aeruginosa PA14 CRISPR2 locus

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1018-Tier1-PhCMV-p2A

Plasmid

#169491

Purpose

Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven 2A sequence of porcine teschovirus-1

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-ccDockerin

Plasmid

#72200

Purpose

MBP-ccDockerin expression plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

Insert

Clostridium Cellulolyticum Type I Dockerin

Tags

His-Tag and Maltose Binding Protein

Expression

Bacterial

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCS105

Plasmid

#174830

Purpose

Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

Depositor

Carol Greider

Article

Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

Insert

11R Unique

Tags

GAL4 5XUAS

Expression

Bacterial

Mutation

Changing TEL11R to a unique end

Promoter

lac promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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