We narrowed to 8,708 results for: sgRNA

1. px459-Flag-Mcm2 targeting sgRNA

   Plasmid

   #186937

   Purpose

   Genomic targeting of Flag tag at Mcm2 N-terminal

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

   Plasmid

   #161924

   Purpose

   To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting RDH11 exon 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 330-BFP-hCYP3A4-enhancer-R-sgRNA

   Plasmid

   #176841

   Purpose

   Targeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer right boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 330-cherry-hCYP3A4-enhancer-L-sgRNA

   Plasmid

   #176840

   Purpose

   Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer left boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330.puro sgRNA KGA Stop1

   Plasmid

   #110405

   Purpose

   CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNTI733 pRPR1(TetO)-RPC31-sgRNA

   Plasmid

   #164913

   Purpose

   For yeast genomic integration of sgRNA against RPC31

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   pRPR1(TetO)-RPC31-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPR1

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTI732 pRPR1(TetO)-HTS1-sgRNA

   Plasmid

   #164912

   Purpose

   For yeast genomic integration of sgRNA against HTS1

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   pRPR1(TetO)-HTS1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPR1

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 sgRNA sCRNA 2.0 (GB2461)

   Plasmid

   #160593

   Purpose

   Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA sCRNA 2.0
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-5U6-sgRNAs-hsyn-EGFP

   Plasmid

   #112213

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   sgRNAs
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid

    #133791

    Purpose

    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St1Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V1 sgRNA architecture from Carter et al. biorxiv …

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid

    #133792

    Purpose

    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    St3Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Muller et al. Molecular T…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

    Plasmid

    #133343

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid

    #133345

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_blaA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330 Gatad2a sgRNA (for flox targeting)

    Plasmid

    #110815

    Purpose

    Used with pNTK Gatad2a flox allele targeting construct in mouse cells

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

    Plasmid

    #114730

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

    Plasmid

    #114728

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

    Plasmid

    #133341

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

    Plasmid

    #108871

    Purpose

    Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Inserts

    5xU6:sgRNA
    Cas9-t2A-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and hsp70

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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