We narrowed to 2,730 results for: PTS;

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pFRT/TO/HIS/FLAG/HA-YTHDF2

Plasmid

#38089

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

YTHDF2 YTH domain family, member 2 (YTHDF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAM1303

Plasmid

#40243

Depositor

Susan Golden

Article

Kulkarni et al Mol Microbiol. 1997 Jun;24(6):1131-42.

Insert

Sp/Sm Resistance, neutral site I integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Mutation

see attached file

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCI118-SiT-Cas12a-[Ind]

Plasmid

#128406

Purpose

Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_003

Plasmid

#192505

Purpose

For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES2_EGFP_KCNQ1

Plasmid

#173161

Purpose

For mammalian expression of KCNQ1 and creating variants

Depositor

Al George

Article

Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

Insert

KCNQ1 (KCNQ1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV CMV-TERRA

Plasmid

#111375

Purpose

TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA polyA

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn deltaSH3

Plasmid

#16034

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Delta SH3: deletion of amino acids 76-141

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn S129E

Plasmid

#36050

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein S129E (SNCA Human)

Expression

Bacterial

Mutation

S129E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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HaloTag-sec61b

Plasmid

#186959

Purpose

Express sec61b connected with N-terminus HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Sec61 (SEC61B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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sfGFP-Peroxisomes-2

Plasmid

#54601

Purpose

Localization: Peroxisomes, Excitation: 485, Emission: 507

Depositor

Michael Davidson

Insert

sfGFP-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Article

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Tags

His

Expression

Bacterial

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
LaM8-AK74

Plasmid

#159594

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at AK74

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn deltaSH3

Plasmid

#16034

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Delta SH3: deletion of amino acids 76-141

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-sec61b

Plasmid

#186959

Purpose

Express sec61b connected with N-terminus HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Sec61 (SEC61B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pKDT17

Plasmid

#27503

Depositor

Peter Greenberg

Article

Pearson et al Proc Natl Acad Sci U S A. 1994 Jan 4. 91

Insert

LasR, LasB::LacZ fusion (lasR Pseudomonas aeruginosa)

Expression

Bacterial

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only