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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,916 results for: FAST

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Showing: 1341 - 1360 of 1916 results

  1. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234440
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Publication
    Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234440-plasmid-map-sequence-id-465720

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  4. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid
    #174081
    Purpose
    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.
    Depositor
    Ryan Mehl
    Publication
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    Tags
    NES-Flag
    Expression
    Mammalian
    Mutation
    L270G, Y271L, N311G, C313A
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174081-plasmid-map-sequence-id-345715

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  8. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Thomas Cech
    Publication
    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86843-plasmid-map-sequence-id-174395

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  9. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234450
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Publication
    Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234450-plasmid-map-sequence-id-465729

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  10. pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

    Plasmid
    #182009
    Purpose
    CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines
    Depositor
    Kai Johnsson
    Publication
    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IgKchL_HA_STf_myc_PDGFRtmb
    Expression
    Mammalian
    Mutation
    Fast mutation on SNAP-tag E30R
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    182009-plasmid-map-sequence-id-355921

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  11. pCMV iGluu

    Plasmid
    #105832
    Purpose
    Fast indicator for extracellular glutamate imaging
    Depositor
    Katalin Torok
    Publication
    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iGluSnFR S72T variant
    Tags
    Myc and PDGFR
    Expression
    Mammalian
    Mutation
    Changed Ser 72 to Thr
    Promoter
    CMV
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    105832-plasmid-map-sequence-id-211677

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  15. pCI syn iGluu

    Plasmid
    #106122
    Purpose
    Fast indicator for synaptic glutamate imaging (synapsin promoter)
    Depositor
    Katalin Torok
    Publication
    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iGluSnFR S72T variant
    Tags
    Myc and PDGFR
    Expression
    Mammalian
    Mutation
    Changed Ser 72 to Thr
    Promoter
    synapsin
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106122-plasmid-map-sequence-id-211683

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  16. pAAV.hSynap.iAChSnFR-Venus-NULL

    Plasmid
    #137953
    Purpose
    Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter
    Depositor
    Loren Looger
    Publication
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (yellow version, non-binding variant)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    cpSFGFP replaced with cpSFVenus; Y140A binding si…
    Promoter
    hSynapsin
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137953-plasmid-map-sequence-id-271684

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  18. pcDNA5/FRT/TO_TOMM20-STf

    Plasmid
    #182002
    Purpose
    CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines
    Depositor
    Kai Johnsson
    Publication
    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20-STf
    Expression
    Mammalian
    Mutation
    Fast mutation on SNAP-tag E30R
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    182002-plasmid-map-sequence-id-356088

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  19. pCMV iGluf

    Plasmid
    #105833
    Purpose
    Fast indicator for extracellular glutamate imaging
    Depositor
    Katalin Torok
    Publication
    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iGluSnFR E25D variant
    Tags
    Myc and PDGFR
    Expression
    Mammalian
    Mutation
    Changed Glu 25 to Asp
    Promoter
    CMV
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    105833-plasmid-map-sequence-id-211679

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  20. pIA028

    Plasmid
    #200419
    Purpose
    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
    Depositor
    Rut Carballido-López
    Publication
    Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016
    Type
    Empty backbone
    Tags
    PAmCherry1
    Expression
    Bacterial
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200419-plasmid-map-sequence-id-405382

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Showing: 1341 - 1360 of 1916 results