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We narrowed to 4,072 results for: golden gate

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Showing: 1341 - 1360 of 4072 results

  1. pPD708 DsRed-Express2-ZF21

    Plasmid
    #138795
    Purpose
    Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF21
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138795-plasmid-map-sequence-id-320733

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  2. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

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  3. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  4. pPD706 DsRed-Express2-ZF13

    Plasmid
    #138793
    Purpose
    Constitutive expression of DsRed-Express2-ZF13 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF13
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138793-plasmid-map-sequence-id-320376

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  5. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  6. C71m

    Plasmid
    #120983
    Purpose
    MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    CinR Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120983-plasmid-map-sequence-id-236042

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  7. pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

    Plasmid
    #242152
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV
    Expression
    Bacterial
    Mutation
    E3K, R146L, A152T, R251L
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242152-plasmid-map-sequence-id-484660

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  8. pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

    Plasmid
    #242153
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC R5 S170 LOV
    Expression
    Bacterial
    Mutation
    A2E, L156I, R251H
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242153-plasmid-map-sequence-id-484652

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  9. pVL1710

    Plasmid
    #239543
    Purpose
    Express Yea4p membrane protein cargo fused to C-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYea4p-2xCrk_SH3-mTurq2-TEVsite-Venus
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239543-plasmid-map-sequence-id-476623

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  10. pVL1708

    Plasmid
    #239542
    Purpose
    Express Yea4p membrane protein cargo fused to N-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    Venus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239542-plasmid-map-sequence-id-476621

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  12. pKS1612

    Plasmid
    #239530
    Purpose
    Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-SKL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239530-plasmid-map-sequence-id-475878

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  13. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239538-plasmid-map-sequence-id-475862

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  14. pAIP4_1YW5

    Plasmid
    #234235
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1YW5 (ESS1 Candida albicans)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234235-plasmid-map-sequence-id-464199

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  15. pAIP4_4HS5

    Plasmid
    #234223
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4HS5
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234223-plasmid-map-sequence-id-464188

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  16. pAIP4_1UKF

    Plasmid
    #234214
    Purpose
    Heterologous protein expression of cysteine protease avirulence protein AvrPphB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UKF
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234214-plasmid-map-sequence-id-464179

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  17. pAIP4_1LU4

    Plasmid
    #234207
    Purpose
    Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1LU4 (mpt53 Mycobacterium tuberculosis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234207-plasmid-map-sequence-id-464172

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  18. pAIP4_1KAF

    Plasmid
    #234209
    Purpose
    Heterologous protein expression of middle transcription regulatory protein motA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KAF (motA Tequatrovirus T4)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234209-plasmid-map-sequence-id-464174

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  19. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

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  20. pAIP4_1TP9

    Plasmid
    #234200
    Purpose
    Heterologous protein expression of peroxiredoxin-2 (Prx) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TP9 (LOC7476296 Populus trichocarpa)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234200-plasmid-map-sequence-id-464165

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Showing: 1341 - 1360 of 4072 results