We narrowed to 2,921 results for: human cdnas

1. pcDNA3-hPC1_MTS-eGFP

   Plasmid

   #108402

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

   Plasmid

   #158953

   Purpose

   Express human RAD51(T309A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed threonine 309 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

   Plasmid

   #69905

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (HIPK3 Fly, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-Flag-H2A K118-119R

   Plasmid

   #63564

   Purpose

   Expression of human H2A with K118R and K119R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 118 and Lysine 119 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3.1-Flag-H2A K13-15R

   Plasmid

   #63563

   Purpose

   Expression of human H2A with K13R and K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 13 and Lysine 15 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

   Plasmid

   #158952

   Purpose

   Express human RAD51(F129A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed phenylalanine 129 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNATM3.1/V5-His TOPO-PKCi-A120E

   Plasmid

   #55687

   Purpose

   expresses constitutively active human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E

   Promoter

   CMV

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

    Plasmid

    #83461

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, R4113A

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-IgL-C99-T4L-rTA

    Plasmid

    #177840

    Purpose

    Tango assay

    Depositor

    Karsten Melcher

    Article

    Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

    Insert

    C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

    Plasmid

    #228532

    Purpose

    A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs10:FRB:HA (SKP1 Human, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

    Plasmid

    #83462

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, W4107A

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

    Plasmid

    #83460

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, S4144D

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO h-PKD1 FLS L4137P-GST

    Plasmid

    #83455

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, L4137P

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO h-PKD1 FLS R4150C-GST

    Plasmid

    #83454

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, R4150C

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2-Cx43-M100/125L-siResist

    Plasmid

    #49855

    Purpose

    Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100 and Methionine 125 mutated to Leuci…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 6A NLRP1 CARD-FLAG WT

    Plasmid

    #163978

    Purpose

    Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

    Plasmid

    #145770

    Purpose

    expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Stop codon at Serine 2013

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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