We narrowed to 8,654 results for: pcDNA3

1. pcDNA3.3-SARS2-B.1.617.2

   Plasmid

   #172320

   Purpose

   expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

   Depositor

   David Nemazee

   Article

   Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

   Insert

   Spike of B.1.617.2 strain (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

   Promoter

   CMV-F

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AKAP79-AKAR4

   Plasmid

   #181957

   Purpose

   Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-AKAR4 (AKAP5 Human)
   

   Tags

   AKAP79, Cerulean, HIS tag, and cpVenus(E172)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ExRai-CKAR2

   Plasmid

   #236096

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Hygro(+)-mscGAS

   Plasmid

   #102607

   Purpose

   Mammalian expression of mouse cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Insert

   cGAS (Cgas Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-mTET2

   Plasmid

   #89735

   Purpose

   Expresses wildtype mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA2-ROC1

   Plasmid

   #19897

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   RING finger protein 1 (ROC1) (RBX1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-RasAR-NES

   Plasmid

   #205567

   Purpose

   Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR-NES (RAF1 Human, Synthetic)
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Flag-Tet2 CD

    Plasmid

    #72219

    Purpose

    Express flag-tagged mouse Tet2 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 2 (Tet2 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET2 catalytic domain.

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1.Gαq-LgB97

    Plasmid

    #134360

    Purpose

    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαq-LgB97 (GNAQ Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-EGFR-V5/HIS

    Plasmid

    #201102

    Purpose

    expression of human EGFR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TLR5-CFP

    Plasmid

    #13019

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 5 (TLR5 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Gα13*-BERKY3

    Plasmid

    #158218

    Purpose

    BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3-TLR2-CFP

    Plasmid

    #13015

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3. Flag-CSL WT

    Plasmid

    #167004

    Purpose

    Mammalian expression of CSL WT

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-FLAG-Rheb

    Plasmid

    #19996

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 (RHEB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-PKA-SPARK

    Plasmid

    #106920

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Expression

    Mammalian

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2/DEST/hTfR-HA

    Plasmid

    #69610

    Purpose

    human transferrin receptor with an HA tag fused at the C-terminus

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    human transferrin receptor (TFRC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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