We narrowed to 8,744 results for: APP

1. pEGFP C1-Eps8 ∆cap/∆bund

   Plasmid

   #74891

   Purpose

   mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

   Insert

   Eps8 (Eps8 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   V689D, L693D, L757A, K759A

   Promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1/TO-myc-BirA*-cCE

   Plasmid

   #112713

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   BirA* (BirA R118G), HIV Rev nuclear localization …

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. GID4-Nluc

   Plasmid

   #200879

   Purpose

   Mammalian expression of human GID4 with C-terminal NanoLuciferase fusion

   Depositor

   Stefan Knapp

   Article

   Owens et al bioRxiv 2023.01.17.524225

   Insert

   GID4-NanoLuciferase (C17orf39 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTKA001

   Plasmid

   #164577

   Purpose

   Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

   Depositor

   Robert Kirchdoerfer

   Article

   Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

   Insert

   nsp7 (ORF1ab )
   

   Use

   Tags

   6xHis, Ek, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTKA002

   Plasmid

   #164578

   Purpose

   Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

   Depositor

   Robert Kirchdoerfer

   Article

   Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

   Insert

   nsp8
   

   Use

   Tags

   6xHis, Ek, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB268

   Plasmid

   #53363

   Purpose

   pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

   Depositor

   Alexander Varshavsky

   Article

   Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

   Inserts

   DHFR (Dhfr Mouse)
   Ub
   Aβ42 (APP Human)
   

   Use

   Tags

   13xMyc, Flag, and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA4/TO-bio-myc-cCE

   Plasmid

   #112711

   Purpose

   For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   HIV Rev nuclear localization signal (NES), Stop c…

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV with Tet repressor binding sites

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZL Neo Myr Flag IKBKE

   Plasmid

   #20504

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. HisGB1-Fusion + CBP TAZ1(340-439)

   Plasmid

   #173762

   Purpose

   Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

   Depositor

   Peter Wright

   Article

   Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

   Inserts

   CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
   CBP (340-439) (Crebbp Mouse)
   

   Use

   Tags

   His6GB1 and thrombin

   Expression

   Bacterial

   Mutation

   fusion of CITED2 and Hif1a

   Promoter

   T7

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAP-COMP-blac-flag-his

    Plasmid

    #111001

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    aminopeptidase P (APP) (PF14_0517 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALE Toolbox

    Plasmid Kit

    #1000000019

    Purpose

    Provides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.

    Depositor

    Feng Zhang

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Mobius Assembly Vector Toolkit

    Plasmid Kit

    #1000000134

    Purpose

    Mobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate parts

    Depositor

    Naomi Nakayama

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

15. AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) (AAV PHP.eB)

    Viral Prep

    #163509-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) (#163509). In addition to the viral particles, you will also receive purified AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) plasmid DNA. hSyn1-driven expression for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Promoter

    Syn

    Tags

    SYFP2

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

16. FRP2008

    Plasmid

    #159312

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    V2del

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FRP2010

    Plasmid

    #159313

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized tSapphire under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    V2del, L231H

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. RelB cFlag pcDNA3

    Plasmid

    #20017

    Depositor

    Stephen Smale

    Insert

    RelB (Relb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.2_mTurquoise2

    Plasmid

    #113723

    Purpose

    Expresses the fluorescent protein mTurquoise2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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