Addgene

We narrowed to 4,404 results for: ARA-2

Showing: 1361 - 1380 of 4404 results

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pET28-His8-aCat(660-885)

Plasmid

#203334

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-885 only

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pROS13

Plasmid

#107927

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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DNMT3ACD-CRY2-EGFP

Plasmid

#82556

Purpose

Encodes DMNT3 under LITE2.0 system

Depositor

Joseph Irudayaraj

Article

Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)
EGFP

Use

AAV

Tags

2A, NLS(alpha-imp), and NLS-VP64

Expression

Mammalian

Mutation

CRY2PHR (N-terminal photolyase homology region do…

Promoter

EF-1alpha

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-ARHGAP26

Plasmid

#69467

Purpose

Expresses ARHGAP26

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

ARHGAP26 (ARHGAP26 Human)

Use

Retroviral

Tags

HA TAG

Expression

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 nu

Plasmid

#44940

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 nu (GRF7 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 epsilon

Plasmid

#44936

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 epsilon (GRF10 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
H6_fold-Z _Gogy

Plasmid

#201828

Purpose

Expresses H6_fold-Z _Gogy in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-Z _Gogy

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H7_fold-K _Mussoc

Plasmid

#201830

Purpose

Expresses H7_fold-K _Mussoc in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H7_fold-K _Mussoc

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-U _Nomur

Plasmid

#201829

Purpose

Expresses H6_fold-U _Nomur in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-U _Nomur

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-C _Rei

Plasmid

#201827

Purpose

Expresses H6_fold-C _Rei in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-C _Rei

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pK031.TRE-Cre (Supernova)

Plasmid

#69136

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

NLS-Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike

Use

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSLS.492

Plasmid

#184957

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22

Use

Tags

Expression

Bacterial

Mutation

rpoB donor T1534C, a1/a2 length extended to 22 bp

Promoter

T7/lac

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb-IRES-eGFP-W

Plasmid

#32519

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Rheb (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCI pHluorin Syt2

Plasmid

#184503

Purpose

Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 2 (Syt2 Mouse)

Use

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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KA1153_pPBCAG-MerCreMer-IN

Plasmid

#124183

Purpose

piggyBac-based expression of the tamoxifen-inducible Cre

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

MerCreMer-IRES-Neo

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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UMG-SP-2_pET29b

Plasmid

#215434

Purpose

Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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KA2628_pGL4-UBCmin-luc2/Tbx3-B'

Plasmid

#124201

Purpose

Luciferase reporter driven by the Tbx3 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Tbx3 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Dapp1-PH pET11a

Plasmid

#71376

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Dapp1-PH (DAPP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 148-273

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only