We narrowed to 4,522 results for: ARA-2

1. Osa-pCAMBIA-1301-STTM529

   Plasmid

   #84225

   Purpose

   Disturb the expression of miR529 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR529
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

2. Gma-pCAMBIA-1301-STTM396

   Plasmid

   #84081

   Purpose

   Disturb the expression of miR396 in soybean

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR396
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gma-pCAMBIA-1301-STTM408

   Plasmid

   #84082

   Purpose

   Disturb the expression of miR408 in soybean

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR408
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

4. Osa-pCAMBIA-1301-STTM397

   Plasmid

   #84207

   Purpose

   Disturb the expression of miR397 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR397
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH004

   Plasmid

   #80401

   Purpose

   Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   CusR (cusR )
   AraC (araC )
   Anti-scaffold
   

   Use

   Synthetic Biology

   Tags

   C-terminal LZx domain

   Expression

   Bacterial

   Available Since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. COVID-SARS2 NSP14/10

   Plasmid

   #159613

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

   Depositor

   Opher Gileadi

   Article

   Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

   Inserts

   Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
   Covid-SARS2 Nsp10
   

   Tags

   His6, TEV cleavage site and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2olig-mCherry

   Plasmid

   #60032

   Purpose

   Expresses a fusion of CRY2PHR E490G with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2PHR E490G (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. B2094_CRY2olig-miRFP

   Plasmid

   #215114

   Purpose

   Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   CRY2olig-miRFP (CRY2 Mustard Weed)
   

   Tags

   miRFP

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-pth2

    Plasmid

    #191428

    Purpose

    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

    Depositor

    Timothy Erickson

    Article

    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

    Insert

    pth2 regulatory region (pth2 Zebrafish)
    

    Use

    Tol2 gateway 5' entry vector

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH016

    Plasmid

    #80400

    Purpose

    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. attB-P7-attP-YFP

    Plasmid

    #195571

    Purpose

    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    

    Use

    Synthetic Biology

    Promoter

    P7

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid

    #160138

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

    Plasmid

    #146691

    Purpose

    Insect Expression of DmHPat-del57-499-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat-del57-499-dsRNAres (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1758G compared to the sequen…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-EGFP-DmHPat-dsRNAres_J

    Plasmid

    #146692

    Purpose

    Insect Expression of DmHPat-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat-dsRNAres (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    two non silent, A479V, G568D and one silent mutat…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-HA-DmHPat-del500-684-dsRNAres_H

    Plasmid

    #146466

    Purpose

    Insect Expression of DmHPat-del500-684-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat-del500-684-dsRNAres (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence gi…

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH015

    Plasmid

    #80398

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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