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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,638 results for: Cas9

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Showing: 1361 - 1380 of 22638 results

  5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118154-plasmid-map-sequence-id-239465

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  6. pCMV-HF-BE3

    Plasmid
    #87439
    Purpose
    Expresses HF-BE3 with C-terminal NLS in mammalian cells
    Depositor
    David Liu
    Publication
    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
    Expression
    Mammalian
    Mutation
    Mammalian codon-optimized Cas9-HF
    Promoter
    CMV
    Available since
    June 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    87439-plasmid-map-sequence-id-298693

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  12. pDHt/sk-PC

    Plasmid
    #92125
    Purpose
    Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR; Trichoderma reesei expression
    Promoter
    Ppdc
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92125-plasmid-map-sequence-id-215731

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  14. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70707-plasmid-map-sequence-id-127770

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  15. GAL4UAS-Luciferase reporter

    Plasmid
    #64125
    Purpose
    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4UAS
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GAL4UAS
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64125-plasmid-map-sequence-id-248531

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  18. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Publication
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99866-plasmid-map-sequence-id-192399

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Showing: 1361 - 1380 of 22638 results