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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Andrew Allen, Ross Bathgate

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH)

Plasmid

#182368

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

glyceraldehyde-3-phosphate dehydrogenase (Gapdh Mouse)

Tags

Pal-Dendra2

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D

Plasmid

#115193

Purpose

Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S293D

Plasmid

#115194

Purpose

Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D/S293D

Plasmid

#115195

Purpose

Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D/S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D/S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 c1655t No Stop

Plasmid

#84892

Purpose

DONOR vector for Gateway cloning of EWSR1 c1655t No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

c1655t No Stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 g1532c No Stop

Plasmid

#84890

Purpose

DONOR vector for Gateway cloning of EWSR1 g1532c No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

g1532c No Stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 No Stop

Plasmid

#84888

Purpose

DONOR vector for Gateway cloning of EWSR1 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

no stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159553

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159546

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 g1750a No Stop

Plasmid

#84894

Purpose

DONOR vector for Gateway cloning of EWSR1 g1750a No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

g1750a No Stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 g1750a Stop

Plasmid

#84893

Purpose

DONOR vector for Gateway cloning of EWSR1 g1750a Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

g1750a

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 c1655t Stop

Plasmid

#84891

Purpose

DONOR vector for Gateway cloning of EWSR1 c1655t Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

c1655t

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cn-VA-NHEJ1-WT

Plasmid

#141339

Purpose

Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1 (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S263E

Plasmid

#141340

Purpose

Lentiviral vector for expression of human NHEJ1-S263E in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S263E (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S263E

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S263A

Plasmid

#141341

Purpose

Lentiviral vector for expression of human NHEJ1-S263A in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S263A (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S263A

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S262E/S263E

Plasmid

#141342

Purpose

Lentiviral vector for expression of human NHEJ1-S262E/S263E in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S262E/S263E (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S262E/S263E

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-S262A/S263A

Plasmid

#141343

Purpose

Lentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1-S262A/S263A (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Mutation

S262A/S263A

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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