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Plasmid#232356PurposeMammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)DepositorInsertOAZ1 derived polyamine sensing module (OAZ1 Human)
UseLentiviralTagseYFP and mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-EYFP
Plasmid#231926PurposeCre-on/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-EYFP
Plasmid#231927PurposeCre-off/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-134-YFP
Plasmid#224882PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-134 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-134PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP135-170-YFP
Plasmid#224878PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP135-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP135-170PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-160-YFP
Plasmid#224886PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-160-YFP
Plasmid#224883PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-M-PRR-Htt97QP4-YFP
Plasmid#224885PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.DepositorInsertM-PRR-Htt97QP4 (HTT Human)
UseTagsYFPExpressionYeastMutationN-PRRPromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-170-YFP
Plasmid#224880PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP161-170-YFP
Plasmid#224876PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.DepositorInsertHTT103QdeltaP161-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdelta161-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP-CAAX-he1.1:YFP
Plasmid#184816PurposeUsed to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTT]-nLuc
Plasmid#199757PurposeElongation reporter construct to quantify elongation of synYFP[CTT]DepositorInsertleuYFP[CTT]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTC]-nLuc
Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-ChR2(H134R)-EYFP
Plasmid#197593PurposeLentiviral transfer vector for the neuronal expression of ChR2(H134R)-EYFP (Channelrhodopsin for the inducing neuronal activity)DepositorInsertChR2(H134R)-EYFP
UseLentiviralTagsExpressionMutationPromoterhSynIAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg6-del36-576-siRNAres_I
Plasmid#146562PurposeMammalian Expression of HsSmg6-del36-576-siRNAresDepositorInsertHsSmg6-del36-576-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg6-del817-1238-siRNAres_I
Plasmid#146564PurposeMammalian Expression of HsSmg6-del817-1238-siRNAresDepositorInsertHsSmg6-del817-1238-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationtwo silent mutations T594C, A699G and two non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg5-del810-846-siRNAres_I
Plasmid#146539PurposeMammalian Expression of HsSmg5-del810-846-siRNAresDepositorInsertHsSmg5-del810-846-siRNAres (SMG5 Human)
UseTagsExpressionMammalianMutationthree non silent mutation A254G (K85R), T980C (L3…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg5-del847-1017-siRNAres_I
Plasmid#146540PurposeMammalian Expression of HsSmg5-del847-1017-siRNAresDepositorInsertHsSmg5-del847-1017-siRNAres (SMG5 Human)
UseTagsExpressionMammalianMutationthree non silent mutation A254G (K85R), T980C (L3…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg5-del13-266-siRNAres_I
Plasmid#146537PurposeMammalian Expression of HsSmg5-del13-266-siRNAresDepositorInsertHsSmg5-del13-266-siRNAres (SMG5 Human)
UseTagsExpressionMammalianMutationtwo non silent mutation, T980C (L327P), T1084C (C…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only