We narrowed to 10,491 results for: aga

1. pCRRNA-G10-R0

   Plasmid

   #101804

   Purpose

   Repress mCherry and GFP independently, with different strengths

   Depositor

   David Bikard, Sven van Teeffelen

   Article

   Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

   Insert

   crRNAs targeting GFP and mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Spy 1049

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPD1R

   Plasmid

   #86969

   Purpose

   Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

   Insert

   PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A/A176R in PTDH

   Promoter

   T7 promoter

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BC-A1-016

   Plasmid

   #78704

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BC-A1-015

   Plasmid

   #78703

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BC-A1-010

   Plasmid

   #78697

   Purpose

   A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BC-A1-008

   Plasmid

   #78695

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BC-A1-007

   Plasmid

   #78694

   Purpose

   A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. shCDK4(1)

   Plasmid

   #73550

   Purpose

   shRNA(1) against CDK4

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pUAST attB BmOr2 (Y464A)

   Plasmid

   #73927

   Purpose

   UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine

   Depositor

   Leslie Vosshall

   Article

   Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

   Insert

   BmOr2 (Y464A)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed 464 Tyrosine to Alanine

   Promoter

   hsp70

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO1-puro-U6-sgRNA-TS4

    Plasmid

    #69236

    Purpose

    U6 driven SpCas9 sgRNA expression for TS4 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHL671

    Plasmid

    #62825

    Purpose

    Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pCon

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pHL1017

    Plasmid

    #62828

    Purpose

    Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    m8::glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET11+PCNA3-CYP119(T214V)

    Plasmid

    #66137

    Purpose

    Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge