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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

Plasmid

#128760

Purpose

Expresses PARN gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PARN sgRNA #2 (PARN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-pro-alphaf-Btl2

Plasmid

#117665

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-pro-alphaf-Btl2

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

Plasmid

#117663

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT2)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala

Plasmid

#179716

Purpose

Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Alpha2-N-18

Plasmid

#55063

Depositor

Michael Davidson

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a-alpha-synuclein

Plasmid

#51486

Purpose

Inducible expression of human alpha-synuclein in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mEmerald-Alpha-V-Integrin-25

Plasmid

#53985

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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GSK3 alpha pMT2

Plasmid

#15896

Depositor

Jim Woodgett

Article

Woodgett et al EMBO J. 1990 Aug . 9(8):2431-8.

Insert

GSK3 alpha (GSK3A Human)

Expression

Mammalian

Available Since

Nov. 1, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pcDL-hu FceRI alpha

Plasmid

#8365

Depositor

Jean Kinet

Article

Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

Insert

human Fc epsilon RI alpha cDNA (FCER1A Human)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pBS598 EF1alpha-EGFPcre

Plasmid

#11923

Purpose

Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-GFP

Plasmid

#66083

Purpose

G protein alpha-s internally tagged with EGFP and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-EGFP (Gnas Rat)

Tags

EGFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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