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pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Tags

6xHis

Expression

Bacterial

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_27F3_hFc

Plasmid

#199619

Purpose

Encodes DARPin-hFc 27F3 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc 27F3

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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cp196V1.2

Plasmid

#52100

Purpose

A improved cpmCherry variant

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

cp196V1.2

Tags

6 His tag

Expression

Bacterial

Promoter

pBAD promotor

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and Citrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1_27B3_hFc

Plasmid

#199618

Purpose

Encodes DARPin-hFc 27B3 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc 27B3

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFd031

Plasmid

#137970

Purpose

Expresses Prochlorococcus marinus MIT9211 Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus MIT9211 ferredoxin 1

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcpmAmetrine

Plasmid

#42170

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

cpmAmetrine173

Tags

6His

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pNHA10

Plasmid

#223554

Purpose

TurboID expression

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

TurboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUdO4c

Plasmid

#210233

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO4a

Plasmid

#210229

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO2a

Plasmid

#210227

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFd022.R2

Plasmid

#137967

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2

Plasmid

#137968

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2

Plasmid

#137969

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJTA022

Plasmid

#137975

Purpose

Inucible expression of Prochlorococcus phage P-SSM2 phage Fd for purification

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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Tubulin-LSSmGFP

Plasmid

#186535

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl2f-mCherry

Plasmid

#164048

Purpose

Optogenetic control of protein localization by PhoCl2f

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2f-mCherry

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citron1

Plasmid

#134305

Purpose

mitochondria-targeted direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-mito-CitronRH

Plasmid

#158547

Purpose

Mitochondria-targeted nonbinding variant of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitronRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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