We narrowed to 3,637 results for: golden gate

1. WT_S1-H1

   Plasmid

   #218392

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

   Plasmid

   #219709

   Purpose

   Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-pSPCH:PhiC31:tUBQ1-GG4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2

   Plasmid

   #219691

   Purpose

   Level 0 to construct history-dependent target T2PB

   Depositor

   Jennifer Nemhauser

   Insert

   GG1-Bxb1P-bbs1-PhiC31P-GG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. WT_S7-H1

   Plasmid

   #218393

   Purpose

   3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. UC16m

   Plasmid

   #121038

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gQi Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. UC17m

   Plasmid

   #121039

   Purpose

   MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gV1 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UCT1m

   Plasmid

   #121041

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   UCT-part guide RNA (Stanley Qi sequence)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C108m

   Plasmid

   #121010

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C114m

   Plasmid

   #121013

   Purpose

   MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   dCas9 with no bbsI or BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C103m

    Plasmid

    #121007

    Purpose

    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

    Depositor

    Richard Murray

    Insert

    mScarlet-I codon optimized with LAA tag
    

    Use

    Synthetic Biology

    Tags

    LAA degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGIG_V5-PPP6C

    Plasmid

    #92013

    Purpose

    Expresses V5-PPP6C, includes IRES-Emerald

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAIP4

    Plasmid

    #233686

    Purpose

    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

    Depositor

    Christopher Bahl

    Insert

    Smt3 (SMT3 )
    

    Use

    Tags

    HisTag

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

    Plasmid

    #228637

    Purpose

    Bacterial expression of the Coxsackievirus 16 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 16 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. ZIKV isolate PRVABC59 NS3 helicase domain

    Plasmid

    #228664

    Purpose

    Bacterial expression of the Zika virus NS3 helicase

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Zika virus NS3 helicase
    

    Use

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAIP4_4WDC

    Plasmid

    #234153

    Purpose

    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4WDC
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid

    #226429

    Purpose

    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

    Depositor

    Alon Goren

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAIP4_3D1B

    Plasmid

    #234230

    Purpose

    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3D1B (tas3 Fission Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAIP4_2ESK

    Plasmid

    #234232

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2ESK (UBE2D2 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAIP4_5F66

    Plasmid

    #234226

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5F66 (PPIA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge