We narrowed to 5,317 results for: golden gate

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pPEPZ-sgRNAclone

Plasmid

#141090

Purpose

This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

Depositor

Jan-Willem Veening

Publication

Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

Insert

mCherry

Use

CRISPR

Expression

Bacterial

Promoter

p3

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ134-tRNA2.0

Plasmid

#158396

Purpose

Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pINEAPLE

Plasmid

#250295

Purpose

Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.

Depositor

Matthew Maurano

Publication

Ordoñez et al bioRxiv 2025.08.13.669251

Insert

SuperfolderGFP

Use

Synthetic Biology; Assembly vector for big-in pay…

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pKG1334_pHarbor-PiggyBac_backbone

Plasmid

#239706

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated int

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1988_pPV1-UbC

Plasmid

#239689

Purpose

BsaI Golden Gate assemby plasmid encoding the UbC promoter

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

UbC promoter

Use

Synthetic Biology

Mutation

Removal of BsaI cut sites

Available since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK084 [CDS_mRFP_PartType9]

Plasmid

#229321

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mRFP_PartType9

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

mRFP

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK024 [Origin_RSF1010_PartType1]

Plasmid

#229261

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

RSF1010 Origin

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA3

Plasmid

#199729

Purpose

Golden Gate entry vector; 3rd gRNA under TaU3 promoter

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU3-sgRNA3 scaffold

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBLsgRNA4

Plasmid

#199730

Purpose

Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C1-sgRNA4 scaffold

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBLsgRNA5

Plasmid

#199731

Purpose

Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C3-sgRNA5 scaffold

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBLsgRNA6

Plasmid

#199732

Purpose

Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.1-sgRNA6 scaffold

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBLsgRNA7

Plasmid

#199733

Purpose

Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.2-sgRNA7 scaffold

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBLsgRNA8

Plasmid

#199734

Purpose

Golden Gate entry vector; 8th gRNA under TaU6 promoter

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU6-sgRNA8 scaffold

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBLggCDS

Plasmid

#199737

Purpose

Golden Gate entry vector for gene of interest cloning

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)

Plasmid

#178988

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1333-C

Plasmid

#174561

Purpose

mitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1333-N

Plasmid

#174560

Purpose

mitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1333-C

Plasmid

#174557

Purpose

Chloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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