We narrowed to 87,646 results for: lias

1. pRV-GN

   Plasmid

   #106373

   Purpose

   creating stable line with GFP and G418 resistance

   Depositor

   Tong-Chuan He

   Article

   Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

   Insert

   eGFP and geneticin/G418 resistance gene
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJHG004

   Plasmid

   #126455

   Purpose

   Expresses dTdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   dTdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Added D343E and D345E to inactivate the enzyme.

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD8a-cNLS-EGFP

   Plasmid

   #86052

   Purpose

   A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CD8a luminal and transmembrane along with two SV4…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

   Plasmid

   #226115

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-107 Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

   Plasmid

   #226109

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-92a Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

   Plasmid

   #226110

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-378a Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

   Plasmid

   #226112

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Use

   Tags

   FLAG, hsa-miR-33b Target Sites, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac1-EcTyr-TAG

   Plasmid

   #228782

   Purpose

   EcTyr aaRS/tRNA system for mammalian ncAA incorporation

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   BstR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGG-PIP32

   Plasmid

   #196770

   Purpose

   Contains RBOHF promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   RESPIRATORY BURST OXIDASE HOMOLOG F (RBOH F Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-AtSLAC1 WT

    Plasmid

    #212946

    Purpose

    Vector used for expression of AtSLAC1 WT in mammalian cell

    Depositor

    Sangho Lee

    Article

    Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

    Insert

    Slow anion channel-associated 1 (OZS1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-AtSLAC1 6D

    Plasmid

    #212947

    Purpose

    Vector used for expression of AtSLAC1 6D in mammalian cell

    Depositor

    Sangho Lee

    Article

    Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

    Insert

    Slow anion channel-associated 1 (OZS1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T62D/S65D/S107D/S124D/S146D/S152D

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRFP2-P2A-HO1-N1

    Plasmid

    #178966

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2-P2A-HO1
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Cas9-T2A-TdT

    Plasmid

    #126424

    Purpose

    Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hum alpha ENAC promoter a25-a23

    Plasmid

    #83427

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

    Insert

    human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    alpha ENAC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTBL210

    Plasmid

    #126451

    Purpose

    Expresses Cas9-XTEN-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTBL469

    Plasmid

    #126458

    Purpose

    Expresses Cas9-15xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    15x Flag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTBL472

    Plasmid

    #126457

    Purpose

    Expresses Cas9-10xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    10x Flag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-AtSLAC1 8D

    Plasmid

    #212948

    Purpose

    Vector used for expression of AtSLAC1 8D in mammalian cell

    Depositor

    Sangho Lee

    Article

    Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

    Insert

    Slow anion channel-associated 1 (OZS1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S59D/T62D/S65D/S86D/S107D/S124D/S146D/S152D

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJHG003

    Plasmid

    #126454

    Purpose

    Expresses Cas9-XTEN-dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-dTdT (DNTT Human, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate TdT.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid

    #170808

    Purpose

    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Inserts

    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Mustard Weed, Synthetic, S. aureus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous mutation in PYL1 to remove XhoI site (…

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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