We narrowed to 6,231 results for: nls

1. pCMV-NLS-MBP-rATOM(WDR5)

   Plasmid

   #209710

   Purpose

   Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   NLS-MBP-rATOM(WDR5)
   

   Use

   Tags

   NLS-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207388

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A, amino acids 10…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PET-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207387

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PET-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207382

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CAGGS-safb∆sap-NLS-3XFLAG-V5

   Plasmid

   #196091

   Purpose

   Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 31-65 (SAP domain) of mouse S…

   Promoter

   CAGGS

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAGGS-safb∆dd1-NLS-3XFLAG-V5

   Plasmid

   #196092

   Purpose

   Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 96-285 (predicted disordered …

   Promoter

   CAGGS

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAGGS-safb∆rrm-NLS-3XFLAG-V5

   Plasmid

   #196093

   Purpose

   Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 428-506 (RRM) of mouse SAFB p…

   Promoter

   CAGGS

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CAGGS-safb∆dd3-NLS-3XFLAG-V5

    Plasmid

    #196094

    Purpose

    Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 638-925 (predicted disordered…

    Promoter

    CAGGS

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid

    #196095

    Purpose

    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Contains only AA 619-925 (NLS and predicted disor…

    Promoter

    CAGGS

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT-ChemoG-NAD-NLS3x

    Plasmid

    #193822

    Purpose

    CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG-NAD-NLS3x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

    Plasmid

    #193828

    Purpose

    CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoL-NAD-NLS3x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA

    Plasmid

    #183802

    Purpose

    AAV vector to express nuclear localized dsRed-Express from CaMKIIa promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    dsRed-Express
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. L0-I11 PhiC31-NLS GG1-GG4

    Plasmid

    #195894

    Purpose

    Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    PhiC31 integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L0-I12 PhiC31-NLS GG2-GG4

    Plasmid

    #195895

    Purpose

    Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    PhiC31 integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-EF1a-ArgSen (-)P2A (-)NLS

    Plasmid

    #184690

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)P2A (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1a-ArgSen (-)P2A (-)NLS

    Plasmid

    #184699

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)P2A (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

    Plasmid

    #182126

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

    Plasmid

    #182121

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge