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We narrowed to 6,025 results for: plasmid dna

Showing: 1361 - 1380 of 6025 results

  1. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid
    #181748
    Purpose
    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Type
    Empty backbone
    Use
    Substrate plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181748-plasmid-map-sequence-id-354677

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  2. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

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  4. pSL3927 (pDonor(plasmid)_Pse)

    Plasmid
    #200905
    Purpose
    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    Mini Tn7016 (pTarget NGS)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200905-plasmid-map-sequence-id-393162

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  6. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  8. dCas9 plasmid

    Plasmid
    #100091
    Purpose
    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3XFlag-NLS-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100091-plasmid-map-sequence-id-197565

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  11. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-403308

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  20. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid
    #101233
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 2 variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    G582A, V583A, E584A, D585A and N588A
    Promoter
    T7
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101233-plasmid-map-sequence-id-194633

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Showing: 1361 - 1380 of 6025 results