We narrowed to 1,627 results for: sp1

1. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FL MALT1 WT in pET29b-Strep

   Plasmid

   #48968

   Purpose

   Expresses Full length wild type MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET21 Catalytic Domain of MALT1

   Plasmid

   #48970

   Purpose

   Expresses catalytic domain of MALT1 in e.coli AA 329-566

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 (MALT1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains AA 329-566

   Promoter

   T7

   Available since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 V5 mDispatched WT 2x HA

   Plasmid

   #126410

   Purpose

   Expression of V5 Dispatched WT HA in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   m-Dispatched1 (Disp1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   V5 iserted between A106 and A107

   Promoter

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINS-USP21 (USP, aa 196-565)

   Plasmid

   #61585

   Purpose

   Expresses human USP21 (USP domain) in E. coli.

   Depositor

   David Komander

   Article

   Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

   Insert

   USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Deleted aa 1-195.

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-hAR1-660

   Plasmid

   #89079

   Purpose

   mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids

   Depositor

   Elizabeth Wilson

   Article

   Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

   Insert

   hAR-1-660 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 1-660 (contains exons A,B,C and part of D)

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-hAR-L26A,F27A

   Plasmid

   #89084

   Purpose

   mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR FxxLF mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-L26A,F27A

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-hAR-F23A

   Plasmid

   #89089

   Purpose

   mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR-FxxLF mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-F23A

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pRK5-HA-mp1

   Plasmid

   #42329

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   mp1 (LAMTOR3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. TetO-FUW-Rfx4

    Plasmid

    #61544

    Purpose

    Expresses Rfx4 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Rfx4 (Rfx4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

11. N-Myc-53BP1 D1521A pLPC-Puro

    Plasmid

    #19837

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    p53 Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    changed Asp1521 to Ala

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pSG5-hAR(16)

    Plasmid

    #89120

    Purpose

    mammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al Mol Endocrinol. 2002 Feb;16(2):287-300.

    Insert

    hAR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    internal EcoRI site removed

    Promoter

    SV40

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

13. GAL-AR-1-503

    Plasmid

    #89093

    Purpose

    mammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Langley et al J Biol Chem. 1995 Dec 15;270(50):29983-9

    Insert

    hAR aa 1-503 (AR Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pHAGE2 GFP-Atl1

    Plasmid

    #86679

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 AMSH 1-178

    Plasmid

    #160050

    Purpose

    Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSH residues 1-178 (STAMBP Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-178

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRR-AR-5Z

    Plasmid

    #23058

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. GST-AR-1-333

    Plasmid

    #104194

    Purpose

    Expresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-333 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2 Smad1 EVE GM

    Plasmid

    #27997

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    Human Smad1 EVE Gsk3 mutant (SMAD1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S463E S465E S210A T202A S191A S198A

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

19. VP16-hAR

    Plasmid

    #89092

    Purpose

    mammalian expression of full-length human androgen receptor with added N-terminal VP16 activation domain

    Depositor

    Elizabeth Wilson

    Article

    Langley et al J Biol Chem. 1998 Jan 2;273(1):92-101.

    Insert

    hAR (AR Human)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pCINeoFlag ALIX; I318A

    Plasmid

    #89867

    Purpose

    Expresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutated isoleucine 318 to alanine

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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