We narrowed to 37,762 results for: tat

1. pSC2

   Plasmid

   #91180

   Purpose

   T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNAs targeting NCR genes
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-IARS2-H112A/P115A

   Plasmid

   #232338

   Purpose

   Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Putative isoleucine binding deficiency mutation H…

   Promoter

   CMV

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-IARS2-K664A/K667A

   Plasmid

   #232339

   Purpose

   Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Putative tRNA binding deficiency mutation K664A A…

   Promoter

   CMV

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-CH111-QES.c13.L544Y-754*

   Plasmid

   #123264

   Purpose

   Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-AD8-QES.i08.c04-754*

   Plasmid

   #123258

   Purpose

   Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (AD8) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-SHIV327C-HuA10-QES.i09.c07

   Plasmid

   #123269

   Purpose

   Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   SHIV327C-Hu A10 Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations V208M, …

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-20b-T7-Ntx-K49

   Plasmid

   #58715

   Purpose

   Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic Acid 49 to Lysine

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. G protein alpha-q-GFP

   Plasmid

   #66080

   Purpose

   This G protein alpha-q construct contains internal insertions of GFP and the EE epitope

   Depositor

   Catherine Berlot

   Article

   Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

   Insert

   G protein alpha-q-EE-YFP (Gnaq Mouse)
   

   Use

   Tags

   GFP was inserted internally into the alpha-q sequ…

   Expression

   Mammalian

   Mutation

   BamHI and SacI sites in alpha-q were removed with…

   Promoter

   CMV

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hSyn Marina-T2A-nls-mCherry

   Plasmid

   #85843

   Purpose

   genetically-encoded fluorescent voltage indicator

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   GEVI Marina
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   hSyn1

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid

    #55797

    Purpose

    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. G protein alpha-q-CFP

    Plasmid

    #66081

    Purpose

    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

    Depositor

    Catherine Berlot

    Article

    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

    Insert

    G protein alpha-q-EE-CFP (Gnaq Mouse)
    

    Use

    Tags

    CFP was inserted internally into the alpha-q sequ…

    Expression

    Mammalian

    Mutation

    BamHI and SacI sites in alpha-q were removed with…

    Promoter

    CMV

    Available since

    July 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid

    #102422

    Purpose

    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    Tet1 catalytic domain mutant (Tet1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1620Y and D1622A mutations in Tet1 catalytic dom…

    Promoter

    TetO-CMV

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3172

    Plasmid

    #144648

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3173

    Plasmid

    #144649

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiGuide-puro_Panc480-MT7

    Plasmid

    #200941

    Purpose

    Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    multiplexed sgRNA array
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-BG505.SOSIP-QES.i03.c01-8his

    Plasmid

    #111846

    Purpose

    Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Use

    Tags

    8his purification tag and CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-BG505.SOSIP-QES.i03.c01-TM

    Plasmid

    #111845

    Purpose

    Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Use

    Tags

    CD5 leader sequence and TM helix from MHC class I

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAD104_Asn152Ser_pCMV6Entry-hIRG1

    Plasmid

    #124877

    Purpose

    Expresses hCAD Asp152Ser mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Asn152 to Ser

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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