Addgene

We narrowed to 605 results for: GI

Showing: 121 - 140 of 605 results

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SpyCatcher002-oHept

Plasmid

#124671

Purpose

Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHept

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEM705 Gso (Cys)

Plasmid

#109375

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Use

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Use

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEM705 Gst (Cys)

Plasmid

#109376

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Use

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gE2

Plasmid

#11621

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E

Use

Tags

His

Expression

Insect

Mutation

deleted amino acids 391-550

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
Halo-ATG16L1 HRD

Plasmid

#207537

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-p62 HRD

Plasmid

#207550

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-P62

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper TR G414C

Plasmid

#207612

Purpose

U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

Telomerase RNA G414C

Use

Tags

Expression

Mammalian

Mutation

Promoter

U1

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-ATG2A HRD

Plasmid

#207540

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG2A locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-Sec61B

Plasmid

#207553

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-Sec61B

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 SNAP-Sec61B

Plasmid

#207580

Purpose

Homologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible SNAPTag-Sec61B

Use

Tags

SNAPTAG

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 LAMP1-mNeonGreen HRD

Plasmid

#207549

Purpose

Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible LAMP1-mNeonGreen

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-LC3B HRD

Plasmid

#207551

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-LC3B

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-GABARAPL1 HRD

Plasmid

#207552

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-GABARAPL1

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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SNAP-LC3 HRD

Plasmid

#207545

Purpose

Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences

Use

Tags

SNAPtag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-ATG5 HRD

Plasmid

#207542

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG5 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-LC3B HRD

Plasmid

#207539

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human LC3B locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin

Plasmid

#141308

Purpose

Mammalian expression of FLAG-tagged Tapasin

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C (CYCS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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