We narrowed to 3,550 results for: golden gate

1. pQGG005

   Plasmid

   #231332

   Purpose

   BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI-ELT4 counterselectable site for BpiI cloning. L2-P15A-Gm-ISceI-ELT4; GG assembly from pOGG006, pOGG009, pNDGG006, pGQ0023, pOGG014. GmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC-HIS6-MBP-TEV-GG

   Plasmid

   #215800

   Purpose

   Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pNIC-HIS6-NUSA-TEV-GG

   Plasmid

   #215798

   Purpose

   Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pNIC-CT-sfGFP-HN-GG

   Plasmid

   #215803

   Purpose

   Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pNIC-HIS6-GST-TEV-GG

   Plasmid

   #215801

   Purpose

   Cloning of target gene with N terminal His6 GST tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pNIC-HIS6-THRX-TEV-GG

   Plasmid

   #215796

   Purpose

   Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pNIC-HIS10-ZBASIC-TEV-GG

   Plasmid

   #215797

   Purpose

   Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pNIC- AVITAG-CTH10STII- -GG

   Plasmid

   #215805

   Purpose

   Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. BsaI-tagRFP-BsaI-pUC-kan

   Plasmid

   #170771

   Purpose

   test plasmid for Golden Gate assembly, encodes fluorescent red protein

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Insert

   tagRFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCB1532H-RFP

    Plasmid

    #101855

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Article

    Pennington et al Figshare (2017)

    Type

    Empty backbone

    Use

    Fungal expression plasmid, tested in magnaporthe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB1532B-RFP

    Plasmid

    #101854

    Purpose

    Golden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Article

    Pennington et al Figshare (2017)

    Type

    Empty backbone

    Use

    Fungal expression plasmid, tested in magnaporthe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNIC-NHSTIIT-AVITAG-GG

    Plasmid

    #215802

    Purpose

    Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pNIC-TolB-NHSTIIT-GG

    Plasmid

    #215792

    Purpose

    Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pNIC-PelB-NH10T-AVITAG-GG

    Plasmid

    #215793

    Purpose

    Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pNIC-HIS6-GST-TEV-AVITAG-GG

    Plasmid

    #215809

    Purpose

    Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pOGG083

    Plasmid

    #114001

    Purpose

    SC module. Beta-glucosidase reporter

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    gusA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOGG039

    Plasmid

    #113996

    Purpose

    SC module. Terminator for T7RNAP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Terminator for T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNIC-OmpA-NHSTIIT-GG

    Plasmid

    #215790

    Purpose

    Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNIC-SufI-NHSTIIT-GG

    Plasmid

    #215791

    Purpose

    Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pNIC-HIS6-SUMO1-AVITAG-GG

    Plasmid

    #215808

    Purpose

    Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge