We narrowed to 3,304 results for: golden gate

1. pNIC-CT-sfGFP-HN-GG

   Plasmid

   #215803

   Purpose

   Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pNIC-HIS6-GST-TEV-GG

   Plasmid

   #215801

   Purpose

   Cloning of target gene with N terminal His6 GST tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pNIC-HIS6-THRX-TEV-GG

   Plasmid

   #215796

   Purpose

   Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pNIC-HIS10-ZBASIC-TEV-GG

   Plasmid

   #215797

   Purpose

   Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCB1532H-RFP

   Plasmid

   #101855

   Purpose

   Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

   Depositor

   Sophien Kamoun

   Article

   Pennington et al Figshare (2017)

   Type

   Empty backbone

   Use

   Fungal expression plasmid, tested in magnaporthe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB1532B-RFP

   Plasmid

   #101854

   Purpose

   Golden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

   Depositor

   Sophien Kamoun

   Article

   Pennington et al Figshare (2017)

   Type

   Empty backbone

   Use

   Fungal expression plasmid, tested in magnaporthe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNIC-NHSTIIT-AVITAG-GG

   Plasmid

   #215802

   Purpose

   Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pNIC-TolB-NHSTIIT-GG

   Plasmid

   #215792

   Purpose

   Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. BsaI-tagRFP-BsaI-pUC-kan

   Plasmid

   #170771

   Purpose

   test plasmid for Golden Gate assembly, encodes fluorescent red protein

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Insert

   tagRFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-PelB-NH10T-AVITAG-GG

    Plasmid

    #215793

    Purpose

    Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pNIC-HIS6-GST-TEV-AVITAG-GG

    Plasmid

    #215809

    Purpose

    Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pOGG083

    Plasmid

    #114001

    Purpose

    SC module. Beta-glucosidase reporter

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    gusA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOGG039

    Plasmid

    #113996

    Purpose

    SC module. Terminator for T7RNAP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Terminator for T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIC-OmpA-NHSTIIT-GG

    Plasmid

    #215790

    Purpose

    Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pNIC-SufI-NHSTIIT-GG

    Plasmid

    #215791

    Purpose

    Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pNIC- AVITAG-CTH10STII- -GG

    Plasmid

    #215805

    Purpose

    Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pNIC-HIS6-SUMO1-AVITAG-GG

    Plasmid

    #215808

    Purpose

    Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pND005

    Plasmid

    #200951

    Purpose

    Yeast two-hybrid destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Yeast two-hybrid

    Tags

    Expression

    Mutation

    Promoter

    GAL4

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGG005

    Plasmid

    #113980

    Purpose

    Level 1 high copy number vector for E. coli expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge