We narrowed to 421 results for: pet-28

1. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

   Plasmid

   #225222

   Purpose

   Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   α-Synuclein
   Aga2
   

   Tags

   GS linker, HA Tag, and c-myc Tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_MBP_RBFOX2

   Plasmid

   #224292

   Purpose

   MBP-RBFOX2 protein purification from bacteria

   Depositor

   Chuan He

   Article

   Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

   Insert

   RNA binding fox-1 homolog 2 (RBFOX2 Human)
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETcon-pGAL1-Aga2-Amyloid β42-c_myc

   Plasmid

   #225223

   Purpose

   Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   Amyloid β42
   Aga2 (AGA2 Budding Yeast)
   

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28 mOct4-POU

   Plasmid

   #206393

   Purpose

   Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192789

   Purpose

   aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody minimizer
   

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192788

   Purpose

   aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody enhancer
   

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. His SUMO TEV R354H SPOP 28-359

   Plasmid

   #197011

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Human)
   

   Tags

   6His-SUMO-TEV

   Expression

   Bacterial

   Mutation

   R354H

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28 MBP-TEV-Rat BCKDK-His

   Plasmid

   #232119

   Purpose

   For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (Bckdk Rat)
   

   Tags

   6 x His, Maltose-Binding Protein (MBP), and Tev S…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 E2 LBD-Tev-E1a-His

    Plasmid

    #232117

    Purpose

    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    DBT (DBT Human)
    

    Tags

    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid

    #232116

    Purpose

    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (BCKDK Human)
    

    Tags

    6 x His, Biotin acceptor peptide (BAP), Maltose-B…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a-mCherry-MED1-IDR

    Plasmid

    #194545

    Purpose

    Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MED1 (MED1 Human)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

    Plasmid

    #110747

    Purpose

    Bacterial expression of human USP30 (construct 13i)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    C77A, F348D, M350S, I353E + insertion deletion: 6…

    Promoter

    T7

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQA-LP574

    Plasmid

    #222953

    Purpose

    Expresses R120A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 120 to Alanine

    Promoter

    T7

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQA-LP578

    Plasmid

    #222957

    Purpose

    Expresses R144S LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 144 to Serine

    Promoter

    T7

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQA-LP577

    Plasmid

    #222956

    Purpose

    Expresses K142S LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Lysine 142 to Serine

    Promoter

    T7

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQA-LP576

    Plasmid

    #222955

    Purpose

    Expresses R141S LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 141 to Serine

    Promoter

    T7

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQA-LP575

    Plasmid

    #222954

    Purpose

    Expresses R36S LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 36 to Serine

    Promoter

    T7

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQA-LP573

    Plasmid

    #222952

    Purpose

    Expresses E40H LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Glutamate 40 to Histidine

    Promoter

    T7

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQA-LP572

    Plasmid

    #222951

    Purpose

    Expresses E40A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Glutamate 40 to Alanine

    Promoter

    T7

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge