We narrowed to 21,156 results for: sequence
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Plasmid#83204Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertSalI/PstI cloning sites
ExpressionPlantAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_zeo insert c-MYC
Plasmid#164636PurposesgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64DepositorInserttarget sequence for c-Myc activation (MYC Human)
UseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
p240 pPr-CREM/Ins
Plasmid#8405PurposeAndrogen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.DepositorInsertprobasin driven CREM with insulators
UseCre/LoxExpressionMammalianMutationmodified CrePromoterProbasin promoterAvailable SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p236 pPr-CREM
Plasmid#8404PurposeExpression of Cre from tissue-specific, androgen-inducible probasin promoter, with chimeric/β-globin intron within the Cre coding sequence.DepositorInsertprobasin driven CREM
UseCre/LoxExpressionMammalianMutationmodified CrePromoterProbasin promoterAvailable SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSPromotervirion-sense LIR and 2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
p201N 1514
Plasmid#47025PurposeContains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencingDepositorInsertsgma-miR1514 recognition sequence
nptII selectable marker
UseRNAiPromoterGmUbi and StUbi-3Available SinceAug. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTR235syn
Plasmid#133464PurposeConsensus sequence of Hop (Humulus lupulus) tandem repeats for cytogeneticsDepositorInsertHuluTR235
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTR400syn
Plasmid#133465PurposeConsensus sequence of Hop (Humulus lupulus) tandem repeats for cytogeneticsDepositorInsertHuluTR400
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTR625syn
Plasmid#133466PurposeConsensus sequence of Hop (Humulus lupulus) tandem repeats for cytogeneticsDepositorInsertHuluTR625
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTR060syn
Plasmid#133462PurposeConsensus sequence of Hop (Humulus lupulus) tandem repeats for cytogeneticsDepositorInsertHuluTR060
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTR120syn
Plasmid#133463PurposeConsensus sequence of Hop (Humulus lupulus) tandem repeats for cytogeneticsDepositorInsertHuluTR120
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRL838
Plasmid#70680PurposeThis vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.DepositorTypeEmpty backboneUseCre/LoxAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-P19-EGFP
Plasmid#196609PurposesiRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeatDepositorInsertp19 + EGFP inverted repeat sequence
Tags6x His tagExpressionBacterialPromoterAraCAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-IRES_ECFP
Plasmid#48354PurposeContributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.DepositorInsertIRES_ECFP
UseGateway entry vectorMutationECFP translation is mediated by an IRES. Contains…PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB1-LOX
Plasmid#154058PurposeLevel 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosTDepositorInsertLoxP-flanked GUS and TtNAM-B1
UseSynthetic BiologyExpressionBacterialMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterZmUbiAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG497 mito-iFAST
Plasmid#130819PurposeExpresses mito-iFAST in mammalian cells (mitochondria labeling)DepositorInsertmito-iFAST
Tagsmyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa-C1V1::FusionRed::Kv2.1
Plasmid#102771Purpose"Activate neurons with 2p stimulation"DepositorInsertC1V1::FusionRed::Kv2.1
UseAAVTagsFusionRed and Kv2.1 traficking domainAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
UCT1m
Plasmid#121041PurposeMoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.DepositorInsertUCT-part guide RNA (Stanley Qi sequence)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only