We narrowed to 24,855 results for: Spr

1. pSplit-SWITCHER1-TF1-dsRED

   Plasmid

   #213044

   Purpose

   A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

   Depositor

   Petr Bartunek

   Article

   Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

   Insert

   Split-SWITCHER1-TF1-dsRED
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   cBH

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSplit-SWITCHER2-TF1-dsRED

   Plasmid

   #213045

   Purpose

   A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

   Depositor

   Petr Bartunek

   Article

   Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

   Insert

   Split-SWITCHER1-TF1-dsRED
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLoxP-EGFP-crRNA-entry

   Plasmid

   #213048

   Purpose

   SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

   Depositor

   Petr Bartunek

   Article

   Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

   Insert

   LoxP-EGFP-MALAT1-triplex
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI5-CasRx-NLS-HA (pKW919)

   Plasmid

   #229047

   Purpose

   Mammalian expression of CasRx-NLS-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI5-CasRx-NES-HA (pKW387)

   Plasmid

   #229048

   Purpose

   Mammalian expression of CasRx-NES-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   NES-HA

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC0x_gRNA_Pos5b_Ptet

   Plasmid

   #179338

   Purpose

   gRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Available Since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMC0x_gRNA_Pos5a_Ptet

   Plasmid

   #179337

   Purpose

   gRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Available Since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFD116

   Plasmid

   #124769

   Purpose

   pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   dcas9
   PpflB sgRNA BsaI
   

   Use

   CRISPR

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSAG1::CAS9-U6::sgUPRT

    Plasmid

    #54467

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    SAG1 5' UTR
    

    Use

    CRISPR; Toxoplasma expression

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

    Plasmid

    #92121

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-pj23119
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQCi-mB2m-sgRNA

    Plasmid

    #154090

    Purpose

    pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA targeting murine B2M locus
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Expression

    Bacterial

    Promoter

    j23119 and ptipA

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQAT22

    Plasmid

    #223394

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT19

    Plasmid

    #223391

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459V2.0-eSpCas9(1.1)

    Plasmid

    #108292

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-SpyCas9

    Plasmid

    #113033

    Purpose

    SpyCas9 expression in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SpyCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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