We narrowed to 30,678 results for: gRNA

1. EED-KO-gRNA-1

   Plasmid

   #131321

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

2. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

   Plasmid

   #136657

   Purpose

   Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:LMG18311 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

   Plasmid

   #136654

   Purpose

   Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:CNRZ1066 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgRNA HBB

   Plasmid

   #128120

   Purpose

   Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA HBB + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgRNA RUNX

   Plasmid

   #128122

   Purpose

   Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA RUNX + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgRNA HEK

   Plasmid

   #128123

   Purpose

   Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA HEK + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-Sox3gRNA-T1

   Plasmid

   #127777

   Purpose

   Chicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox3gRNAT1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-Sox3gRNA-T2

   Plasmid

   #127778

   Purpose

   Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox3gRNAT2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR-nfatc1_sgRNA2

   Plasmid

   #132978

   Purpose

   nfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Insert

   nfatc1 sgRNA2 target sequence
   

   Use

   CRISPR; Sgrna generation vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDR-itga9_sgRNA1

    Plasmid

    #132979

    Purpose

    itga9 sgRNA targeting to "5'-GAATGACGGAGCTCTCTACG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA1 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDR-itga9_sgRNA4

    Plasmid

    #132980

    Purpose

    itga9 sgRNA targeting to "5'-TCCGCTGCCAGCCAGCCGG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA4 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDR-itga9_sgRNA7

    Plasmid

    #132981

    Purpose

    itga9 sgRNA targeting to "5'-CCCTCCAGCCTTCCATATG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA7 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-chiRNA_dCrkgRNA1

    Plasmid

    #131139

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6-chiRNA_dCrkgRNA2

    Plasmid

    #131140

    Purpose

    Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    dCrk gRNA1 (Crk Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLR5-U6-sgRNA-EF

    Plasmid

    #82635

    Purpose

    piggyBac vector for expressing modified sgRNA in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Type

    Empty backbone

    Use

    CRISPR; Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid

    #127908

    Purpose

    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Human 5' HP1beta
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. gRNA-NONO-1

    Plasmid

    #127654

    Purpose

    Knock-out of human NONO (guide only)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO sgRNA (NONO Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTBL251 GG sgRNA

    Plasmid

    #126436

    Purpose

    To target a protospacer with GG at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with GG at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTBL510 CA sgRNA

    Plasmid

    #126430

    Purpose

    To target a protospacer with CA at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with CA at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTBL401 GC sgRNA

    Plasmid

    #126435

    Purpose

    To target a protospacer with GC at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with GC at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge