We narrowed to 5,008 results for: puc

1. pAd388-shLuc

   Plasmid

   #83275

   Purpose

   shRNA targeting luciferace

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA to Luciferase
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   pUC ori

   Available since

   Dec. 2, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTA1cI-T3

   Plasmid

   #193789

   Purpose

   Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Truncated version of Lambda CI, named T3, where the first two codons are deleted
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO-1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KG#797

   Plasmid

   #110916

   Purpose

   Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   unc-129 promoter
   STRN-1 (Sentryn)-GFP
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Use

   Tags

   GFP (contains 3 artificial introns)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KG#920

   Plasmid

   #110937

   Purpose

   Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   mig-13 promoter
   INS-22-Emerald
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKI-cjPLP1e2-P15L-_lox

   Plasmid

   #129756

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   PLP1 (PLP1 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   P15L

   Promoter

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKI-cjPLP1e2-A39T

   Plasmid

   #129752

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   PLP1 (PLP1 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   A39T

   Promoter

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKI-cjPLP1e2-P15L

   Plasmid

   #129753

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   PLP1 (PLP1 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   P15L

   Promoter

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KG#493

   Plasmid

   #110932

   Purpose

   Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

   Depositor

   Kenneth Miller

   Article

   Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

   Inserts

   unc-17 promoter
   unc-54 3' control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KG#94

    Plasmid

    #110931

    Purpose

    Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

    Depositor

    Kenneth Miller

    Article

    Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

    Inserts

    unc-17 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAVS1-Bxb1-LP-TC

    Plasmid

    #183754

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox257
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid

    #99692

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1-phiC31-LP-TC

    Plasmid

    #183755

    Purpose

    AAVS1 targeting construct with the phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMS1932

    Plasmid

    #79781

    Purpose

    High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple259
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple259 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEMS1937

    Plasmid

    #79782

    Purpose

    High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple260
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple260 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pENTR-eGFP-attP(bxb)-*BsdR

    Plasmid

    #183751

    Purpose

    Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBR_attB(bxb)_ccdB_lox

    Plasmid

    #183763

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ccdB
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Use

    Tags

    Lck and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge