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We narrowed to 25,308 results for: Nes

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Showing: 1401 - 1420 of 25308 results

  1. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  2. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61362-plasmid-map-sequence-id-99796

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  3. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

    Enlarge

  4. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

    Enlarge

  5. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

    Enlarge

  6. K980

    Plasmid
    #129762
    Purpose
    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129762-plasmid-map-sequence-id-263587

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  7. K406

    Plasmid
    #129764
    Purpose
    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 406
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129764-plasmid-map-sequence-id-263574

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  8. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  9. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

    Enlarge

  10. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  11. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

    Enlarge

  12. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129761-plasmid-map-sequence-id-422377

    Enlarge

  13. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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Showing: 1401 - 1420 of 25308 results