We narrowed to 14,071 results for: cas9

1. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

   Plasmid

   #107731

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-ChBtW-eSpCas9

   Plasmid

   #133370

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUPD2 dCas9 (GB2664)

   Plasmid

   #160596

   Purpose

   CDS of dCas9 with B3 nomenclature

   Depositor

   Diego Orzaez

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFREE2-eSpCas9-sgRNA

   Plasmid

   #179580

   Purpose

   Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   eSpCas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC148-pCR8-dCas9VP96

   Plasmid

   #48220

   Purpose

   dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP96 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP96

   Mutation

   D10A;H840A

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVMG0173_Cq-Actin5c-Cas9

   Plasmid

   #169345

   Purpose

   Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0173_Cq-Actin5c-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CPIJ009808

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVMG0212_Cq-nanos-Cas9

   Plasmid

   #169348

   Purpose

   Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

   Depositor

   Valentino Gantz

   Article

   Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

   Insert

   pVMG0212_Cq-nanos-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CPIJ011551

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVMG0213_Cq-vasa-Cas9

    Plasmid

    #169347

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0213_Cq-vasa-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ009286

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Intron4-PAFAH1B1

    Plasmid

    #92434

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_UdgX-EE-UdgX-NG-nCas9

    Plasmid

    #163570

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-dCas9-SET7-Hygro

    Plasmid

    #104407

    Purpose

    transient expression of dCas9-SET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    SET7 (SETD7 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-H11r1-2-DD-Cas9

    Plasmid

    #85978

    Purpose

    Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBLO 9.6_bCas9-CP1010

    Plasmid

    #121498

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    p15A

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBLO 9.15_bCas9-CP1249

    Plasmid

    #121500

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    p15A

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO 9.16_bCas9-CP1282

    Plasmid

    #121501

    Purpose

    TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    p15A

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBbdCas9G(RBSmut)_arr2

    Plasmid

    #149578

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbdCas9K(-10TC)_arr2

    Plasmid

    #149584

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbdCas9K(RBSmut)_arr2

    Plasmid

    #149577

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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