We narrowed to 11,784 results for: emb

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Human Transporter Knockout library

Pooled library

#213695

Purpose

Next generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Ferdigg et al Life Sci Alliance. 2024 Nov 22;8(2):e202

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only
pLV-EF1A-LivePAR-Hygro

Plasmid

#176063

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAMBIA_ASX

Plasmid

#98888

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Publication

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

CaMV35S (enhanced)

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NINJ1

Plasmid

#201181

Purpose

Dox-inducible expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Publication

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Promoter

TRE3G

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseC

Plasmid

#89724

Purpose

Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseC (pseC Campylobacter jejuni)

Tags

His8 and TEV

Expression

Bacterial

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only
His8-TEV-PseH

Plasmid

#89725

Purpose

Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseH (pseH Campylobacter jejuni)

Tags

2xHis Gln 5xHis and TEV

Expression

Bacterial

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Publication

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

Lck (MGCVCSSNPELPVAT)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MSCV-SNAP-Smo

Plasmid

#31619

Depositor

Rajat Rohatgi

Publication

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
U6>sgRNA(F+E)

Plasmid

#59986

Purpose

Empty vector for sgRNA cloning (F+E modified backbone)

Depositor

Lionel Christiaen

Publication

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Type

Empty backbone

Use

CRISPR

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLV-EF1A-CD19.28H.28TM.BB.3z

Plasmid

#200679

Purpose

Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Publication

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET24-ISWI-iCBD

Plasmid

#105499

Purpose

Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

Depositor

James Kadonaga

Publication

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

ISWI-iCBD

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pYTK072

Plasmid

#65179

Purpose

Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConRE

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pWarf(+)

Plasmid

#34562

Depositor

Jeff Abramson

Publication

Hsieh et al Protein Sci. 2010 Apr;19(4):868-80.

Insert

HRV 3C-GPA-GFP-8HIS

Expression

Bacterial

Available since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRE:iRFP670-EFS:Cre-2A-GFP

Plasmid

#68477

Purpose

Inducible lentivirus expressing iRFP670, Cre, and GFP

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

iRFP670
Cre-2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EFS and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pmyr GFP

Plasmid

#50528

Depositor

Kenneth Yamada

Insert

membrane marker

Tags

Src myristylation signal

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCoofy3

Plasmid

#43983

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-GST

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCoofy4

Plasmid

#43986

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSheLL

Plasmid

#37319

Depositor

John Schiller

Publication

Buck et al J Virol. 2004 Jan;78(2):751-7.

Insert

BPVL1+L2

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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C1-CFP-Lyn11

Plasmid

#155234

Purpose

membane marker

Depositor

Tobias Meyer

Publication

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 membrane targeted CFP. Membrane control

Tags

CFP

Expression

Mammalian

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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