We narrowed to 2,921 results for: human cdnas

1. pcDNA 3.1 myc-Rab10-mito

   Plasmid

   #208367

   Purpose

   Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   myc-Rab10-mito (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5 FRT TO myc Separase S1226A/ C2029A

   Plasmid

   #59823

   Purpose

   Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S1226A/ C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

   Plasmid

   #205604

   Purpose

   Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinΔGTP-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A, N229A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.3-Nm-dCas9-p300 Core

   Plasmid

   #61365

   Purpose

   encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D16A, D587A, H588A, N611A in Nm Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

   Plasmid

   #115788

   Purpose

   Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   LRP5 (LRP5 Human)
   

   Use

   Tags

   3xflag

   Expression

   Mammalian

   Mutation

   contains silent mutation that creates an internal…

   Promoter

   CMV

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-eGFP-PC1-3HA

   Plasmid

   #108406

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   3HA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-hPC1_4106-eGFP-3HA

   Plasmid

   #108399

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. (318) pcDNA3.1-myc-OGA(1-400)D174N

   Plasmid

   #168096

   Purpose

   Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   myc-OGA(1-400, D174N)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D174N catalytically inactive OGA

   Promoter

   T7/CMV

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

   Plasmid

   #83456

   Purpose

   Human PC1-CTp30 expression cassette with a GST-tag (678)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Amino-acids 4107-4303

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid

    #59826

    Purpose

    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5 FRT TO myc Separase delta 55/S1126A

    Plasmid

    #59825

    Purpose

    Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/S1126A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

    Plasmid

    #83463

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4169

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

    Plasmid

    #83459

    Purpose

    Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, S4144D

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

    Plasmid

    #163979

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3 Flag MFF iso5 S172A

    Plasmid

    #74382

    Purpose

    Flag tagged human S172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 Flag MFF iso5 S155,172A

    Plasmid

    #74383

    Purpose

    Flag tagged human S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    See 155 Ala, Ser 172 Ala (numbering based on MFF …

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3 Flag MFF iso5 S155A

    Plasmid

    #74381

    Purpose

    Flag tagged human S155A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala (numbering based on MFF isoform 1)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1/CALR ins5 (del1-17)-FLAG

    Plasmid

    #204533

    Purpose

    Mammalian expression of human CALR ins5 (del1-17)-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR ins5 (del1-17)-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ins5 (del1-17)

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(-) rat C/EBP alpha

    Plasmid

    #12550

    Depositor

    Peter Johnson

    Article

    Miller et al J Biol Chem. 2003 Apr 25;278(17):15178-8

    Insert

    CCAAT/Enhancer-binding Protein alpha (Cebpa Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

    Plasmid

    #201818

    Purpose

    A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    V5-ER-LBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge