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p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pWPI-GFP-3xFLAG

Plasmid

#201639

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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CAS9PBKS

Plasmid

#68371

Purpose

CbAkt promoter driven Cas9-2A-GFP (from px458)

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

Cas9

Tags

2A-EGFP

Expression

Mammalian

Promoter

CbAkt

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPB9v1

Plasmid

#210028

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-BRD4

Plasmid

#183939

Purpose

Transcriptional co-activator BRD4 labeled by mCherry

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

mCherry-BRD4 (Brd4 Mouse, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-hHSF1 WT

Plasmid

#71724

Purpose

plasmid expressing myc-his tag hHSF1 WT

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

HSF1 (HSF1 Human)

Tags

MYC 6xHIS

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-RPA70

Plasmid

#164231

Purpose

retroviral plasmid for GFP-RPA70

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

RPA70 (RPA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1

Plasmid

#202012

Purpose

Moclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

NbGP41-sfGFP-VP64-GB1

Use

Synthetic Biology; Moclo level 1 vector

Tags

GB1 and sfGFP

Promoter

AtUBI10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[85Q]

Plasmid

#33237

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 85 Glutamines and no…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[30Q]

Plasmid

#33236

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 29 amino acids (27 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLX302 OASL-V5 puro

Plasmid

#158643

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OASL.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OASL (OASL Human)

Use

Lentiviral

Tags

V5

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302 OAS1-V5 puro

Plasmid

#158641

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS1 (OAS1 Human)

Use

Lentiviral

Tags

V5

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-EFS-CasRx-pA

Plasmid

#192488

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[2Q]

Plasmid

#33235

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-L-V5-His

Plasmid

#222848

Purpose

E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

V5-His

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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