164,298 results

1. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Use

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sigma 70 promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

   Plasmid

   #171437

   Purpose

   Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   c-Fos-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPXLd

   Plasmid

   #12258

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV/D374Y-hPCSK9

   Plasmid

   #58379

   Purpose

   Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Insert

   proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D374Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDsRed-attP

   Plasmid

   #51019

   Purpose

   Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

   Depositor

   Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Inserts

   attP
   LoxP-3xP3-DsRed-LoxP
   

   Use

   High copy, amp resistance

   Tags

   Expression

   Mutation

   Promoter

   3xP3 and None

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. ADRA2C-Tango

   Plasmid

   #66218

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA2C (ADRA2C Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHAGE-TO-nmdCas9-3XGFP

   Plasmid

   #64109

   Purpose

   Expression of Nm dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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