We narrowed to 15,874 results for: pET

1. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5787 pET28aSX104B/ pES42

   Plasmid

   #1229

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pET29b-ClbPpep-CHis

   Plasmid

   #48243

   Purpose

   First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP-pep
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   First 375 amino acids of the peptidase, which con…

   Promoter

   T7

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-dH3-dH4

   Plasmid

   #105502

   Purpose

   Co-expression of Drosophila histones H3 and H4

   Depositor

   James Kadonaga

   Article

   Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

   Insert

   dH3-dH4
   

   Available Since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-51b(+)-Caprola_14

   Plasmid

   #194675

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_14
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-51b(+)-Caprola_15

   Plasmid

   #194676

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_15
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-pro-sumo3

   Plasmid

   #25103

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO3 (SUMO3 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Serine 2 to Alanine

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pET28a(His10-PD-1cyto)

   Plasmid

   #177896

   Purpose

   E coli expression of His10 tag fused to human PD-1 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   PD-1 (aa 194-288 only) (PDCD1 Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET14b PHAS-I F113A

   Plasmid

   #15680

   Depositor

   John Lawrence

   Article

   Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

   Insert

   PHAS-I F113A (Eif4ebp1 Rat)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F113A mutation.

   Available Since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFS_0143-pET30-Fusicatenibacter_saccharivorans

    Plasmid

    #116952

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pET-PLS-KP6_rpoA-C

    Plasmid

    #190966

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETa(+) HpCsd3 (42-403)

    Plasmid

    #71285

    Purpose

    Expresses Csd3 from Helicobacter pylori

    Depositor

    SeWon Suh

    Article

    An et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    HP0506 (HP0506 )
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pET51b-NAD-cpLuc

    Plasmid

    #135628

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc sensor
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM-60-NusA-DmBelle_B

    Plasmid

    #145963

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Expression

    Bacterial

    Mutation

    del 155-157, T530A and I685V

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET16b-Trx C35S

    Plasmid

    #36947

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin-1 C35S mutation (TXN Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    cys 35 to serine

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pET30-hDlg PDZ2

    Plasmid

    #21692

    Depositor

    James Baleja

    Article

    Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

    Insert

    hDlg/SAP97 PDZ2 domain (DLG1 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    human hDlg/SAP97 PDZ2 domain (residues 318-405)

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pET-ChE1 v4.5

    Plasmid

    #185755

    Purpose

    Bacterial expression of ChE1 v4.5

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    ChE1 v4.5
    

    Tags

    His10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET SUMO-FL-syt1

    Plasmid

    #213628

    Purpose

    Bacterial expression of SUMO-tagged rat FL-syt1

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt1 (Syt1 Rat)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Tags

    His-T7

    Expression

    Bacterial

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pET-DUET-Prp18

    Plasmid

    #111354

    Purpose

    protein purification

    Depositor

    Christine Guthrie

    Insert

    PRP18
    

    Expression

    Bacterial

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET-His-Tre

    Plasmid

    #123129

    Purpose

    Bacterial expression plasmid for Tre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Tre recombinase
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3992 pET ubcH5

    Plasmid

    #8652

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH5 (UBE2D1 Human)
    

    Expression

    Bacterial

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only