We narrowed to 1,529 results for: Flp

1. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220610

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

   Plasmid

   #220611

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-3x HA-hM3D(Gq)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

   Plasmid

   #220613

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220614

   Purpose

   Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

   Plasmid

   #51248

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907,980,1006 mutated to A…

   Promoter

   EF1a

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220608

   Purpose

   Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

   Plasmid

   #51249

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 980,1006 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

   Plasmid

   #51247

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

   Plasmid

   #220609

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-FRT-Rev-TdTomato

    Plasmid

    #191203

    Purpose

    FlpO dependent TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    TdTomato
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CAG-FRT-Rev-3xGFP

    Plasmid

    #191204

    Purpose

    FlpO dependent GFP expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    3X GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid

    #115518

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    eGFP
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZ:F3-CAGGS GPHTK-F

    Plasmid

    #112666

    Purpose

    Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    eGFP
    

    Use

    Gene targeting vector for integration at the s1 s…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA5_miniTurbo-C12orf49

    Plasmid

    #155111

    Purpose

    transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112668

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA5_C12orf49-miniTurbo

    Plasmid

    #155112

    Purpose

    transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    C12orf49 (SPRING1 Human)
    

    Tags

    3x Flag and miniTurbo

    Expression

    Mammalian

    Promoter

    CMV/TO inducible promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZ:F3-P CAGGS tdTPH-F

    Plasmid

    #112667

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5 rtTA BirA eIF4G

    Plasmid

    #158789

    Purpose

    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag and BirA*

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-TRE-FRT-2xtTA

    Plasmid

    #191205

    Purpose

    Flpo dependent tTA expression under the control of TRE promotor

    Depositor

    Janelia Viral Tools

    Article

    Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

    Insert

    2xtTA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5 rtTA BirA eIF4G +MIC

    Plasmid

    #158790

    Purpose

    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon)
    

    Tags

    3xFlag and BirA*

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge